In vitro activities of antimicrobial combinations against planktonic and biofilm forms of Stenotrophomonas maltophilia

doi:10.3389/fmicb.2023.1186669

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Title:	In vitro activities of antimicrobial combinations against planktonic and biofilm forms of Stenotrophomonas maltophilia
Authors:	Su, Bo-An Chen, Chi-Chung Chen, Hung-Jui Lai, Hsin-Yu Tsai, Chia-Hung Lai, Chih-Cheng Tang, Hung-Jen Chao, Chien-Ming
Contributors:	Chi Mei Med Ctr, Dept Internal Med, Div Infect Dis Chia Nan Univ Pharm & Sci, Dept Pharm , Tainan, Taiwan;[Chen, Chi-Chung Chia Nan Univ Pharm & Sci, Dept Hlth & Nutr Chang Jung Christian Univ, Dept Biosci Technol Chi Mei Med Ctr, Div Hosp Med, Dept Internal Med Natl Sun Yat Sen Univ, Coll Med, Sch Med Chi Mei Med Ctr, Dept Med Chi Mei Med Ctr, Dept Intens Care Med
Keywords:	Stenotrophomonas maltophilia levofloxacin fosfomycin ceftazidime-avibactam tigecycline
Date:	2023
Issue Date:	2024-12-25 11:04:54 (UTC+8)
Publisher:	FRONTIERS MEDIA SA
Abstract:	ObjectivesTo investigate the in vitro activity of antibiotic combinations against Stenotrophomonas maltophilia isolates and their associated biofilms. MethodsThirty-two S. maltophilia clinical isolates with at least twenty-five different pulsotypes were tested. The antibacterial activity of various antibiotic combinations against seven randomly selected planktonic and biofilm-embedded S. maltophilia strains with strong biofilm formation was assessed using broth methods. Extraction of bacterial genomic DNA and PCR detection of antibiotic resistance and biofilm-related genes were also performed. ResultsThe susceptibility rates of levofloxacin (LVX), fosfomycin (FOS), tigecycline (TGC) and sulfamethoxazole-trimethoprim (SXT) against 32 S. maltophilia isolates were 56.3, 71.9, 71.9 and 90.6%, respectively. Twenty-eight isolates were detected with strong biofilm formation. Antibiotic combinations, including aztreonam-clavulanic (ATM-CLA) with LVX, ceftazidime-avibactam (CZA) with LVX and SXT with TGC, exhibited potent inhibitory activity against these isolates with strong biofilm formation. The antibiotic resistance phenotype might not be fully caused by the common antibiotic-resistance or biofilm-formation gene. ConclusionS. maltophilia remained resistant to most antibiotics, including LVX and & beta;-lactam/& beta;-lactamases; however, TGC, FOS and SXT still exhibited potent activity. Although all tested S. maltophilia isolates exhibited moderate-to-strong biofilm formation, combination therapies, especially ATM-CLA with LVX, CZA with LVX and SXT with TGC, exhibited a higher inhibitory activity for these isolates.
Relation:	Frontiers in Microbiology, v.14, Article 1186669
Appears in Collections:	[Offices] 456

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