Orthosiphon aristatus (Blume) Miq. Extracts attenuate Alzheimer-like pathology through anti-inflammatory, anti-oxidative, and β-amyloid inhibitory activities

doi:10.1016/j.jep.2023.117132

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Title:	Orthosiphon aristatus (Blume) Miq. Extracts attenuate Alzheimer-like pathology through anti-inflammatory, anti-oxidative, and β-amyloid inhibitory activities
Authors:	Chiang, Kuang-Hsing Cheng, Tain-Junn Kan, Wei-Chih Wang, Hsien-Yi Li, Jui-Chen Cai, Yan-Ling Cheng, Chia-Hui Liu, Yi-Chien Chang, Chia-Yu Chuu, Jiunn-Jye
Contributors:	Taipei Med Univ, Taipei Heart Inst Taipei Med Univ Hosp, Div Cardiol Taipei Med Univ Hosp, Cardiovasc Res Ctr Taipei Med Univ, Coll Med, Sch Med, Dept Internal Med Natl Taiwan Univ, Grad Inst Biomed Elect & Bioinformat Chi Mei Med Ctr, Dept Neurol Dept Occupat Med Chi Mei Med Ctr, Dept Geriatr & Geront Chi Mei Med Ctr, Div Nephrol Chung Hwa Univ Med Technol, Dept Med Lab Sci & Biotechnol Chia Nan Univ Pharm & Sci, Coll Leisure & Recreat Management, Dept Sport Management Wei Gong Mem Hosp, Pharm Dept Southern Taiwan Univ Sci & Technol, Coll Engn, Dept Biotechnol & Food Technol Southern Taiwan Univ Sci & Technol, Ctr Gen Educ
Keywords:	Orthosiphon aristatus (OA) Alzheimer's disease (AD) Beta-amyloid(A beta) Neurofibrillary tangles (NFT)
Date:	2024
Issue Date:	2024-12-25 11:04:39 (UTC+8)
Publisher:	ELSEVIER IRELAND LTD
Abstract:	Ethnopharmacological relevance: Orthosiphon aristatus (Blume) Miq. (OA) is a traditional folk-herb, which is usually used to treat acute and chronic nephritis, epilepsy, cystitis, and other diseases. Phenols and flavonoids are the main active compound compounds of OA, with proven anti-inflammatory and antioxidant activities.Aims of this study: Based on evidenced therapeutic activities, we aimed to investigate the impact of OA on Alzheimer's disease (AD) which is the most common age-related neurodegenerative disease, and the pathological features include accumulation of beta-amyloid (A beta) and neurofibrillary tangles (NFT). Materials and methods: OA was extracted with water, methanol, chloroform, and ethyl acetate, and determined its total flavonoid and phenolic contents. Initially, A beta 1-42 based cytotoxicity was induced in BV2 cells and C6 cells to investigate the therapeutic impact of OA therapy by MTT, RT-PCR, Western blot, and ELISA. Further, A beta 1-42 Oligomer (400 pmol)-induced AD mice model was established to evaluate the impact of OA extract on improving learning and memory impairment.Results: The results showed that the extract of OA could increase cell survival, inhibit the expression of TNF-alpha, IL6, IL-1 beta, COX-2, and iNOS, and increase BDNF levels. We infer that the OA extract may attenuate A beta-induced cytotoxicity by retarding the production of inflammatory-related factors. In the animal behavior test, the number of mice entering darkroom and the time of arriving at the platform were significantly reduced, indicating the learning and memory-improving ability of OA extract.Conclusions: These findings imply that the OA ethanolic extract demonstrated an improving effect on memory and hence could serve as a potential therapeutic target for the treatment of neurodegenerative diseases like AD.
Relation:	Journal of Ethnopharmacology, v.320, Article 117132
Appears in Collections:	[Offices] 456

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