Cloning, identification, and functional characterization of novel prophenoloxidases (ShproPO) from the freshwater crab Sinopotamon henanense in response to cadmium exposure and Aeromonas hydrophila infection

doi:10.1016/j.fsi.2023.108565

Chia Nan University of Pharmacy & Science Institutional Repository > 行政單位 > 456 > Item 310902800/34842

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/34842

標題:	Cloning, identification, and functional characterization of novel prophenoloxidases (ShproPO) from the freshwater crab Sinopotamon henanense in response to cadmium exposure and Aeromonas hydrophila infection
作者:	Liang, Yue Bao, Minnan Lang, Lang Wang, Lu Wang, Shijie Chen, Chien M. Chu, Ka-Hou Wang, Lan
貢獻者:	Shanxi Univ, Sch Life Sci Shenzhen Technol Univ Chia Nan Univ Pharm & Sci, Dept Environm Resources Management Chinese Univ Hong Kong, Sch Life Sci
關鍵字:	Sinopotamon henanense Prophenoloxidases Prophenoloxidase activation system Cadmium Aeromonas hydrophila
日期:	2023
上傳時間:	2024-12-25 11:04:22 (UTC+8)
出版者:	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
摘要:	Prophenoloxidase (proPO) is essential in the prophenoloxidase-activating system (proPO-AS) which is important for defense against foreign infection in crustaceans. However, most studies have focused on expression in the presence of a single pathogenic bacterium, and very few have addressed the presence of environmental contaminants simultaneously, such as cadmium (Cd) and Aeromonas hydrophila. Our study aimed to investigate the function of proPO in the freshwater crab Sinopotamon henanense and the changes in its expression by Cd and infection of A. hydrophila. A novel proPO from the hemocytes of S. henanense (ShproPO) was found in this research, the full-length cDNA of ShproPO was 2620 bp of encoding a protein of 678 amino acids containing three typical hemocyanin domains. The ShproPO protein could be found in both the granular (GHc) and the semi-granular hemocytes (SGHc). The ShproPO mRNA was found to be abundantly expressed in hemocytes and could be influenced by A. hydrophila infection. These results indicate that ShproPO could be involved in the antibacterial process. Further research found that low concentrations of Cd could promote its expression after infection with A. hydrophila. Therefore, it was hypothesized that Cd disrupted the response of crabs to A. hydrophila infection. Subsequently, PO enzyme activity was found to be significantly reduced through in vivo RNA interference with ShproPO, and the results suggested that ShproPO is likely to be a key enzyme in the melanization response. Finally, ShproPO was found to significantly enhance the phagocytosis of A. hydrophila-infected hemocytes by in vitro recombination, confirming that ShproPO is involved in hemocyte-mediated melanization and phagocytosis. Our findings reveal completely new insight into the immunotoxicity of Cd and the immune function of ShproPO in S. henanense.
關聯:	Fish & Shellfish Immunology, v.134, Article 108565
显示于类别:	[行政單位] 456

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html		0Kb	HTML	3	检视/开启

在CNU IR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....