Anisomeles indica Extracts and Their Constituents Suppress the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro

doi:10.3390/ijms242015062

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 藥學系(所) > 期刊論文 > Item 310902800/34825

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/34825

標題:	Anisomeles indica Extracts and Their Constituents Suppress the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro
作者:	Chen, Yu-Ru Jiang, Wen-Ping Deng, Jeng-Shyan Chou, Ya-Ni Wu, Yeh-Bin Liang, Hui-Ju Lin, Jaung-Geng Huang, Guan-Jhong
貢獻者:	China Med Univ, Coll Chinese Med, Dept Chinese Pharmaceut Sci & Chinese Med Resource Chia Nan Univ Pharm & Sci, Dept Pharm Asia Univ, Dept Food Nutr & Hlth Biotechnol Arjil Pharmaceut LLC
關鍵字:	SARS-CoV-2 Anisomeles indica ovatodiolide anisomlic acid apigenin ACE2 TMPRSS2
日期:	2023
上傳時間:	2024-12-25 11:04:07 (UTC+8)
出版者:	MDPI
摘要:	Coronavirus disease 2019 (COVID-19), stemming from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has had a profound global impact. This highly contagious pneumonia remains a significant ongoing threat. Uncertainties persist about the virus's effects on human health, underscoring the need for treatments and prevention. Current research highlights angiotensin-converting enzyme 2 (ACE2) and transmembrane protease serine 2 (TMPRSS2) as key targets against SARS-CoV-2. The virus relies on ACE2 to enter cells and TMPRSS2 to activate its spike protein. Inhibiting ACE2 and TMPRSS2 expression can help prevent and treat SARS-CoV-2 infections. Anisomeles indica (L.) Kuntze, a medicinal plant in traditional Chinese medicine, shows various promising pharmacological properties. In this study, ethanolic extracts of A. indica were examined both in vivo (250 and 500 mu M) and in vitro (500 mu M). Through Western blotting analysis, a significant reduction in the expression levels of ACE2 and TMPRSS2 proteins was observed in HepG2 (human hepatocellular carcinoma) cells and HEK 293T (human embryonic kidney) cell lines without inducing cellular damage. The principal constituents of A. indica, namely, ovatodiolide (5 and 10 mu M), anisomlic acid (5 and 10 mu M), and apigenin (12.5 and 25 mu M), were also found to produce the same effect. Furthermore, immunohistochemical analysis of mouse liver, kidney, and lung tissues demonstrated a decrease in ACE2 and TMPRSS2 protein expression levels. Consequently, this article suggests that A. indica and its constituents have the potential to reduce ACE2 and TMPRSS2 protein expression levels, thus aiding in the prevention of SARS-CoV-2 infections.
關聯:	International Journal of Molecular Sciences, v.24, n.20, Article 15062
显示于类别:	[藥學系(所)] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
ijms242015062.pdf		3388Kb	Adobe PDF	6	检视/开启
index.html		0Kb	HTML	7	检视/开启

在CNU IR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....