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| 標題: | Designable Poly(methacrylic Acid)/Silver Cluster Ring Arrays as Reflectance Spectroscopy-Based Biosensors for Label-Free Plague Diagnosis |
| 作者: | Chen, Chih-Wei
Chen, Shih-Hsun
Huang, Chih-Feng
Chen, Jem-Kun |
| 貢獻者: | Chi Mei Med Ctr, Dept Surg, Div Neurosurg
Chia Nan Univ Pharm & Sci, Inst Ind Safety & Disaster Prevent, Coll Sustainable Environm, Dept Occupat Safety & Hlth
Natl Taiwan Univ Sci & Technol, Dept Mat & Sci Engn
Natl Yang Ming Chiao Tung Univ, Dept Mech Engn
Natl Chung Hsing Univ, I Ctr Adv Sci & Technol iCAST, Dept Chem Engn |
| 關鍵字: | silver clusters
poly(methacrylic acid) brush
plague
reflectance spectra |
| 日期: | 2023 |
| 上傳時間: | 2024-12-25 11:03:56 (UTC+8) |
| 出版者: | MDPI |
| 摘要: | A hole array was fabricated via photolithography to wet the bottoms of holes using oxygen plasma. Amide-terminated silane, a water immiscible compound before hydrolysis, was evaporated for deposition on the plasma-treated hole template surface. The silane compound was hydrolyzed along the edges of circular sides of the hole bottom to form a ring of an initiator after halogenation. Poly(methacrylic acid) (PMAA) was grafted from the ring of the initiator to attract Ag clusters (AgCs) as AgC-PMAA hybrid ring (SPHR) arrays via alternate phase transition cycles. The SPHR arrays were modified with a Yersinia pestis antibody (abY) to detect the antigen of Yersinia pestis (agY) for plague diagnosis. The binding of the agY onto the abY-anchored SPHR array resulted in a geometrical change from a ring to a two-humped structure. The reflectance spectra could be used to analyze the AgC attachment and the agY binding onto the abY-anchored SPHR array. The linear range between the wavelength shift and agY concentration from 30 to 270 pg mL(-1) was established to obtain the detection limit of similar to 12.3 pg mL(-1). Our proposed method provides a novel pathway to efficiently fabricate a ring array with a scale of less than 100 nm, which demonstrates excellent performance in preclinical trials. |
| 關聯: | Polymers, v.15, n.8, Article 1919 |
| 顯示於類別: | [職業安全衛生系(含防災所)] 期刊論文
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