Lipid Accumulation in Blastocystis Increases Cell Damage in Co-Cultured Cells

doi:10.3390/microorganisms11061582

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標題:	Lipid Accumulation in Blastocystis Increases Cell Damage in Co-Cultured Cells
作者:	Liao, Chen-Chieh Chen, Chun-Hsien Shin, Jyh-Wei Lin, Wei-Chen Chen, Chien-Chin Chu, Chun-Ting
貢獻者:	Natl Cheng Kung Univ, Coll Med, Inst Microbiol & Immunol, Dept Parasitol Natl Cheng Kung Univ, Coll Med, Dept Physiol Natl Cheng Kung Univ, Coll Med, Dept Microbiol & Immunol Natl Cheng Kung Univ, Inst Basic Med Sci, Coll Med Chia Yi Christian Hosp, Ditmanson Med Fdn, Dept Pathol Chia Nan Univ Pharm & Sci, Dept Cosmet Sci Natl Cheng Kung Univ, Coll Biosci & Biotechnol, Dept Biotechnol & Bioind Sci Natl Chung Hsing Univ, Rong Hsing Res Ctr Translat Med Chia Yi Christian Hosp, Ditmanson Med Fdn, Dept Surg, Div ColoRectal Surg
關鍵字:	Blastocystis ST7-B lipid pathogenesis Caco-2 cell
日期:	2023
上傳時間:	2024-12-25 11:03:55 (UTC+8)
出版者:	MDPI
摘要:	Blastocystis hominis is an intestinal protozoan that is often neglected, despite causing abdominal pain and diarrhea. Previous research has demonstrated that lipids can be synthesized by B. hominis or can accumulate in growth medium, but their function and mechanisms in the pathogenesis of Blastocystis remain unclear. Our study found that lipid-rich Blastocystis ST7-B can increase inflammation and disrupt Caco-2 cells more than the same parasite without the lipovenoes supplement. Additionally, the cysteine protease of Blastocystis, a virulence factor, is upregulated and has higher activity in lipid-rich Blastocystis. In order to better understand the effects of lipids on Blastocystis pathogenesis, we treated lipid-lowering pravastatin during Blastocystis ST7-B culturing with a lipovenoes supplement, which decreased the lipid levels of the Blastocystis and reduced the Blastocystis-induced inflammation and cell disruption of Caco-2 cells. We also analyzed the fatty acid composition and possible synthesis pathway in Blastocystis ST7-B, finding significantly higher ratios of arachidonic acid, oleic acid, and palmitic acid than in the other lipid components in lipid-rich Blastocystis ST7-B. These results suggest that lipids play a significant role in the pathogenesis of Blastocystis and provide important information on the molecular mechanisms of and potential treatments for Blastocystis infection.
關聯:	Microorganisms, v.11, n.6, Article 1582
顯示於類別:	[行政單位] 456

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