English  |  正體中文  |  简体中文  |  Items with full text/Total items : 18055/20253 (89%)
Visitors : 24592256      Online Users : 399
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34706


    標題: PD-L1 expressed from tumor cells promotes tumor growth and invasion in lung cancer via modulating TGF-β1/SMAD4 expression
    作者: Chen, Ming-Jenn
    Wang, Yao-Chen
    Wang, Lee
    Shen, Ching-Ju
    Chen, Chih-Yi
    Lee, Huei
    貢獻者: Chi Mei Hospital
    Chia Nan University of Pharmacy & Science
    Chung Shan Medical University
    Chung Shan Medical University Hospital
    Chung Shan Medical University
    Kaohsiung Medical University
    Kaohsiung Medical University Hospital
    Chung Shan Medical University
    Chung Shan Medical University Hospital
    Taipei Medical University
    關鍵字: 16/18 e6 oncoprotein
    human-papillomavirus
    b7-h1 expression
    infiltrating lymphocytes
    clinical-significance
    egfr mutations
    carcinoma
    survival
    overexpression
    progression
    日期: 2022
    上傳時間: 2023-12-11 14:06:15 (UTC+8)
    出版者: WILEY
    摘要: Background Programmed death ligand-1 (PD-L1) has a known association with the prognosis of human cancers because of its ability to alter tumor immune surveillance via its interaction with PD-1. We questioned whether expression of PD-L1 in tumor cells could directly promote tumor growth and invasiveness in non-small cell lung cancer (NSCLC). Methods Real-time reverse transcription-polymerase chain reaction (RT-PCR) was performed to evaluate PD-L1 messenger RNA (mRNA) expression in lung tumors. The prognostic value of PD-L1 mRNA was assessed by Cox regression model. Transcriptional regulation of PD-L1 by human papillomavirus (HPV) 16/18 E6 oncoprotein or by epidermal growth factor receptor (EGFR) mutation in lung cancer cells was examined by Western blot and luciferase reporter assay. The cell growth and invasion were evaluated by colony formation, soft agar growth, and Boyden chamber assay. Results The PD-L1 mRNA levels showed a positive association with HPV 16/18 E6 oncoprotein and with EGFR mutation in 223 surgically resected NSCLC patients. The prognostic significance of PD-L1 was more commonly observed in patients with high PD-L1/E6 positive and high PD-L1/EGFR mutant tumors. Mechanistically, upregulation of PD-L1 transcription by E6 or mutant EGFR occurred largely through the ERK-C/EBP beta-TLR4-NF-kappa B cascade. PD-L1 promotes the efficacy of colony formation, soft agar growth, and cell invasion. PD-L1 upregulates BAG-1 to reduce transforming growth factor (TGF)-beta 1 expression, and the decrease in SMAD4 because of TGF-beta 1 occurs through the p53/microRNA (miR)-224 axis. The decreases in TGF-beta 1 and SMAD4 are responsible for PD-L1-mediated cell invasiveness. Conclusion Induction of PD-L1 by E6 oncoprotein or mutant EGFR through the ERK-C/EBP beta-TLR4-NF-kappa B cascade may promote tumor growth and invasiveness in NSCLC because of decreasing TGF-beta 1 and SMAD4 expression.
    關聯: THORACIC CANCER, v.13, n.CB2, pp.CC2, pp.-,
    Appears in Collections:[行政單位] 123

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML112View/Open


    All items in CNU IR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback