Auranofin induces urothelial carcinoma cell death via reactive oxygen species production and synergy with cisplatin

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Title:	Auranofin induces urothelial carcinoma cell death via reactive oxygen species production and synergy with cisplatin
Authors:	Chen, San-Yuan Chao, Chun-Nun Huang, Hsin-Yi Fang, Chiung-Yao
Contributors:	Chia-Yi Christian Hospital Department of Sports Management, Chia Nan University of Pharmacy & Science Chia-Yi Christian Hospital Asia University Taiwan Chia-Yi Christian Hospital
Keywords:	bladder-cancer molecular-mechanisms anticancer activity ros apoptosis checkpoint generation resistance induction stress
Date:	2022
Issue Date:	2023-12-11 14:04:06 (UTC+8)
Publisher:	SPANDIDOS PUBL LTD
Abstract:	Urothelial carcinoma (UC) is one of the most common cancer types of the urinary tract. UC is associated with poor 5-year survival rate, and resistance to cisplatin-based therapy remains a challenge for invasive bladder cancer treatment. Therefore, there is an urgent need to develop new drugs for advanced UC therapy. Auranofin (AF) was developed over 30 years ago for the treatment of rheumatoid arthritis and has been reported to exert an antitumor effect by increasing the level of reactive oxygen species (ROS) in cancer cells. The aim of the present study was to examine the effects of AF on cancer cell proliferation, cell cycle and apoptosis, either alone or in combination with cisplatin. AF induced cell death in two separate cell lines, HT 1376 and BFTC 909, in a concentration- and time-dependent manner by inducing cell cycle arrest. However, the distribution of cells in different phases of the cell cycle differed between the two cell lines, with G(0)/G(1) cell cycle arrest in HT 1376 cells and S phase arrest in BFTC 909 cells. In addition, AF induced apoptosis in HT 1376, as well as redox imbalance in both HT 1376 and BFTC 909 cells. Cell viability was rescued following treatment with N-acetyl-L-cysteine, a ROS scavenger. Furthermore, AF treatment synergistically increased the cytotoxicity of HT 1376 and BFTC 909 cells when combined with cisplatin treatment. These findings suggest that AF may represent a potential candidate drug against UC and increase the therapeutic effect of cisplatin.
Relation:	ONCOLOGY LETTERS, v.23, n.2, pp.61
Appears in Collections:	[Dept. of Sports Management] Periodical Articles

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