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| 標題: | Alpinumisoflavone Exhibits the Therapeutic Effect on Prostate Cancer Cells by Repressing AR and Co-Targeting FASN- and HMGCR-Mediated Lipid and Cholesterol Biosynthesis |
| 作者: | Basavaraj, Praveenkumar
Ruangsai, Phakkhathorn
Hsieh, Po-Fan
Jiang, Wen-Ping
Bau, Da-Tian
Huang, Guan-Jhong
Huang, Wen-Chin |
| 貢獻者: | China Medical University Taiwan
China Medical University Taiwan
China Medical University Taiwan
China Medical University Hospital - Taiwan
CDepartment of Pharmacy, Chia Nan University of Pharmacy & Science
China Medical University Taiwan
China Medical University Hospital - Taiwan
Asia University Taiwan
China Medical University Taiwan |
| 關鍵字: | fatty-acid synthase
androgen receptor
apoptosis
progression
lipogenesis
inhibition
activation
growth |
| 日期: | 2022 |
| 上傳時間: | 2023-12-11 14:02:09 (UTC+8) |
| 出版者: | MDPI |
| 摘要: | Prostate cancer (PCa) is the most common cancer in men, and this has been mainly noticed in Western and Asian countries. The aggregations of PCa and castration-resistant PCa (CRPC) progression are the crucial causes in the mortality of patients without the effective treatment. To seek new remedies for the lethal PCa diseases is currently an urgent need. In this study, we endeavored to investigate the therapeutic efficacy of alpinumisoflavone (AIF), a natural product, in PCa. LNCaP (androgen- sensitive) and C4-2 (CRPC) PCa cells were used. An MTT-based method, soft agar colony forming assay, biological progression approaches were applied to determine cell viability, migration, and invasion. A fatty acid quantification kit, a cholesterol detection kit and oil red O staining were conducted to analyze the intracellular levels of lipids and cholesterols. Apoptosis assays were also performed. AIF reduced cell viability, migration, and invasion in PCa cells. The expression of androgen receptor (AR), fatty acid synthase (FASN), and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) was substantially inhibited by AIF treatment in PCa cells. Furthermore, by inhibiting FASN and HMGCR expression, AIF decreased the amounts of intracellular fatty acids, cholesterols, and lipid droplets in PCa cells. Significantly, through coordinated targeting FASN- and HMGCR-regulated biosynthesis and the AR axis, AIF activated the caspase-associated apoptosis in PCa cells. These results collectively demonstrated for the first time the potential of AIF as a novel and attractive remedy and provided an alternative opportunity to cure PCa malignancy. |
| 關聯: | LIFE-BASEL, v.12, n.11, 1769 |
| 顯示於類別: | [藥學系(所)] 期刊論文
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