Oroxylin-A and its phosphonate derivative potentiate eNOS/NO-mediated relaxation and attenuate vasoconstrictor-induced contraction in the mouse aorta

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Title:	Oroxylin-A and its phosphonate derivative potentiate eNOS/NO-mediated relaxation and attenuate vasoconstrictor-induced contraction in the mouse aorta
Authors:	Tseng, Tzu-Ling Ho, Wen-Yueh Huang, Po-Jui Liao, Jin-Zhi Lee, Kuan-Han
Contributors:	Buddhist Tzu Chi General Hospital Hualien Tzu Chi Hospital Tzu Chi University of Science & Technology Chia Nan University of Pharmacy & Science Kaohsiung Medical University Chia Nan University of Pharmacy & Science Chia Nan University of Pharmacy & Science Chia Nan University of Pharmacy & Science
Keywords:	nitric-oxide memory impairment channels inhibition flavonoids
Date:	2022
Issue Date:	2023-12-11 14:01:55 (UTC+8)
Publisher:	JAPANESE PHARMACOLOGICAL SOC
Abstract:	Oroxylin-A (OroA), a flavonoid isolated from Scutellariae baicalensis, alleviates cardiovascular dysfunction. Several procedures for synthesizing OroA have been developed but show low production yield and regioselectivity. We synthesized OroA from baicalin using a one-pot reaction to increase its overall yield. We also determined the chemical properties and mechanism of action of the synthesized OroA and OroA phosphate diethyl ester (OroA-OET) in vascular function. The induction of vascular reactivity by OroA and OroA-OET was evaluated using blood vessel myography and biochemical analysis to assess nitric oxide synthase-mediated nitric oxide production in mouse aortic arteries. OroA and OroA-OET (0.1-30 mM) induced sustained vasorelaxation, which was partly mediated by the endothelium in isolated normal arteries pre-contracted with phenylephrine. OroA and OroA-OET significantly attenuated vasoconstrictors-induced contractile responses. Dilation effects were blocked by the non-selective nitric oxide synthase inhibitor N (omega)-nitro-L-arginine methyl ester but not by tetraethylammonium or 1H(1,2,4)oxadiazolo [4,3-a]quinoxalin-1-one. Notably, preincubation with OroA and OroA-OET potentiated acetylcholine-induced relaxation and endothelial nitric oxide production in the arteries with the endothelium. OroA and OroA-OET protected against cardiovascular dysfunction. The synthesis and lead compounds used not only improved the yield of OroA from natural sources but also potentially regulated vascular tone.(c) 2022 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/ licenses/by-nc-nd/4.0/).
Relation:	JOURNAL OF PHARMACOLOGICAL SCIENCES, v.150, n.CB2, pp.CC2, pp.-,
Appears in Collections:	[Offices] 123

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