Species identification and antifungal susceptibility of uncommon blood yeast isolates

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標題:	Species identification and antifungal susceptibility of uncommon blood yeast isolates
作者:	Li, Ming-Chi Tang, Hung-Jen Wu, Chi-Jung Wang, Shin-Wei Su, Shu-Li Liu, Wei-Lun Ko, Wen-Chien Chen, Yee-Chun
貢獻者:	National Cheng Kung University National Cheng Kung University Hospital National Cheng Kung University Chi Mei Hospital Department of Health and Nutrition, Chia Nan University of Pharmacy & Science National Health Research Institutes - Taiwan National Cheng Kung University National Cheng Kung University Hospital National Cheng Kung University National Cheng Kung University Hospital Fu Jen Catholic University Fu Jen Catholic University Hospital Fu Jen Catholic University National Cheng Kung University National Taiwan University National Taiwan University Hospital National Taiwan University
關鍵字:	desorption ionization-time mass-spectrometry systems broth microdilution candida-haemulonii bruker biotyper fungemia profile agents ms
日期:	2022
上傳時間:	2023-12-11 14:01:33 (UTC+8)
出版者:	ELSEVIER TAIWAN
摘要:	Background/Purpose: Accurate identification of Candida species is increasingly important in the era of emergence of Candida auris. We aimed to compare the identification performance of two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems (Vitek MS and Bruker biotyper MS) and an oligonucleotide array for uncommon blood yeast isolates and demonstrate the susceptibilities among those isolates. Method: Candida species isolates from blood culture other than Candida albicans, Candida parapsilosis, Candida tropicalis, Candida glabrata, and Candida krusei identified by biochemical methods were collected from multiple hospitals and further identified by an oligonucleotide array based on the internal transcribed spacer-1 (ITS-1) and ITS-2 sequences of the rRNA genes, Vitek MS and Bruker biotyper MS. The minimal inhibitory concentrations (MICs) of these clinical isolates were determined by the Sensititre YeastOne (SYO) system. Results: Among 136 isolates, Candida guilliermondii was most common (52, 38.2%), followed by C. lusitaniae (13, 9.6%) and C. haemulonii (12, 8.8%). The oligonucleotide array, Vitek MS and Bruker biotyper MS correctly identified 89.7% (122), 90.4% (123), and 92.6% (126) of these isolates, respectively. Elevated minimal inhibitory concentrations (MICs) of fluconazole were observed for C. haemulonii (MIC90: 256 mg/L), and C. guilliermondii (MIC90: 16 mg/L) with 28.4% of uncommon Candida isolates with MIC >= 8 mg/L. Conclusions: For uncommon Candida species, the unmet need for current databases of two commercial MALDI-TOF MS systems is highlighted, and the oligonucleotide array may serve as a supplement. Copyright (C) 2021, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC.
關聯:	JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION, v.55, Issue 1, pp.130-137
顯示於類別:	[保健營養系(所) ] 期刊論文

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