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| 標題: | Species identification and antifungal susceptibility of uncommon blood yeast isolates |
| 作者: | Li, Ming-Chi
Tang, Hung-Jen
Wu, Chi-Jung
Wang, Shin-Wei
Su, Shu-Li
Liu, Wei-Lun
Ko, Wen-Chien
Chen, Yee-Chun |
| 貢獻者: | National Cheng Kung University
National Cheng Kung University Hospital
National Cheng Kung University
Chi Mei Hospital
Department of Health and Nutrition, Chia Nan University of Pharmacy & Science
National Health Research Institutes - Taiwan
National Cheng Kung University
National Cheng Kung University Hospital
National Cheng Kung University
National Cheng Kung University Hospital
Fu Jen Catholic University
Fu Jen Catholic University Hospital
Fu Jen Catholic University
National Cheng Kung University
National Taiwan University
National Taiwan University Hospital
National Taiwan University |
| 關鍵字: | desorption ionization-time
mass-spectrometry systems
broth microdilution
candida-haemulonii
bruker biotyper
fungemia
profile
agents
ms |
| 日期: | 2022 |
| 上傳時間: | 2023-12-11 14:01:33 (UTC+8) |
| 出版者: | ELSEVIER TAIWAN |
| 摘要: | Background/Purpose: Accurate identification of Candida species is increasingly important in the era of emergence of Candida auris. We aimed to compare the identification performance of two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems (Vitek MS and Bruker biotyper MS) and an oligonucleotide array for uncommon blood yeast isolates and demonstrate the susceptibilities among those isolates. Method: Candida species isolates from blood culture other than Candida albicans, Candida parapsilosis, Candida tropicalis, Candida glabrata, and Candida krusei identified by biochemical methods were collected from multiple hospitals and further identified by an oligonucleotide array based on the internal transcribed spacer-1 (ITS-1) and ITS-2 sequences of the rRNA genes, Vitek MS and Bruker biotyper MS. The minimal inhibitory concentrations (MICs) of these clinical isolates were determined by the Sensititre YeastOne (SYO) system. Results: Among 136 isolates, Candida guilliermondii was most common (52, 38.2%), followed by C. lusitaniae (13, 9.6%) and C. haemulonii (12, 8.8%). The oligonucleotide array, Vitek MS and Bruker biotyper MS correctly identified 89.7% (122), 90.4% (123), and 92.6% (126) of these isolates, respectively. Elevated minimal inhibitory concentrations (MICs) of fluconazole were observed for C. haemulonii (MIC90: 256 mg/L), and C. guilliermondii (MIC90: 16 mg/L) with 28.4% of uncommon Candida isolates with MIC >= 8 mg/L. Conclusions: For uncommon Candida species, the unmet need for current databases of two commercial MALDI-TOF MS systems is highlighted, and the oligonucleotide array may serve as a supplement. Copyright (C) 2021, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. |
| 關聯: | JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION, v.55, Issue 1, pp.130-137 |
| 顯示於類別: | [保健營養系(所) ] 期刊論文
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