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    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34622

    標題: Construction and characterization of an engineered recombinant Rhodopseudomonas palustris to remove Cd2+, Zn2+ and Cu2+
    作者: Jia, Qing-Yun
    Chen, Chien-Min
    Li, Bao-Zhen
    Wang, Lan
    貢獻者: Shanxi University
    Department of Environmental Resources Management, Chia Nan University of Pharmacy & Science
    Shanxi University of Chinese Medicine
    Shanxi University
    關鍵字: crab sinopotamon-henanense
    heavy-metal toxicity
    biosorption behavior
    cadmium biosorption
    日期: 2022
    上傳時間: 2023-12-11 14:01:11 (UTC+8)
    摘要: In this study, we used a photosynthetic bacteria as a host to construct a novel engineered bacterium Rhodop-seudomonas palustris expressed with the metallothionein (MT) from the freshwater crab (Sinopotamon honanense), and to investigate its removal and biosorption capacity for Cd, Zn and Cu in medium and the possible mechanism (s). The results showed that: (i) the MT expressed recombinant was constructed successfully; (ii) the order of heavy metal sensitivity of the recombinant was Cd > Cu > Zn, and the maximum biosorption capacities calculated by Langmuir model were 224.22, 233.64 and 99.8 mg/g, respectively. Compared to the wild-type, the recombinant has doubled biosorption capacity to Cd and Cu with higher sorption rates and was less influenced by different culturing factors (time, metal concentration and pH), which allowed it rapid removal of 92.1% of 25 mg/L Cd within 30 mins; (iii) the recombinant exhibited more diverse biosorption patterns, and the proportion of its intracellular uptake was significantly (P < 0.05) increased 10% under different conditions; (iv) the functional groups on the cell membrane of the recombinant involved in the metal biosorption were more selective, and the synthesis of metal nanoparticles might be reused as optoelectronic materials for recycling. Based on the findings, we concluded that under various culturing conditions, the recombinant can remove the metals examined more successfully and efficiently. Therefore, as a novel strain, the recombinant has great potential for application in the removal and recovery of metals from wastewater or other polluted water sources.
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