Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/34590
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    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34590


    Title: Characterization of a silent azaphilone biosynthesis gene cluster in Aspergillus terreus NIH 2624
    Authors: Sun, Wei-Wen
    Li, Chi-Ying
    Chiang, Yi-Ming
    Lin, Tzu-Shyang
    Warren, Shauna
    Chang, Fang-Rong
    Wang, Clay C. C.
    Contributors: University of Southern California
    Graduate Institute of Pharmaceutical Science, Chia Nan University of Pharmacy & Science
    Kaohsiung Medical University
    University of Southern California
    Keywords: heterologous expression
    secondary metabolites
    tet-on
    discovery
    pathway
    system
    chemistry
    genomics
    Date: 2022
    Issue Date: 2023-12-11 13:59:27 (UTC+8)
    Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
    Abstract: Filamentous fungal secondary metabolites are an important source of bioactive components. Genome sequencing of Aspergillus terreus revealed many silent secondary metabolite biosynthetic gene clusters presumed to be involved in producing secondary metabolites. Activation of silent gene clusters through overexpressing a pathway-specific regulator is an effective avenue for discovering novel fungal secondary metabolites. Replace-ment of the native promoter of the pathway-specific activator with the inducible Tet-on system to activate the taz pathway led to the discovery of a series of azaphilone secondary metabolites, among which azaterrilone A (1) was purified and identified for the first time. Genetic deletion of core PKS genes and transcriptional analysis further characterized the taz gene cluster to consist of 16 genes with the NR-PKS and the HR-PKS collaborating in a convergent mode. Based on the putative gene functions and the characterized compounds structural infor-mation, a biosynthetic pathway of azaterrilone A (1) was proposed.
    Relation: FUNGAL GENETICS AND BIOLOGY, v.160, May 2022, 103694
    Appears in Collections:[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles

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