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| 標題: | Protodioscin inhibits bladder cancer cell migration and growth, and promotes apoptosis through activating JNK and p38 signaling pathways |
| 作者: | Chen, Yuan-Ru
Wang, Shu-Chi
Huang, Shu-Pin
Su, Chia-Cheng
Liu, Po-Len
Cheng, Wei-Chung
Chuu, Chih-Pin
Chen, Jen-Kun
Bao, Bo-Ying
Lee, Cheng Hsueh
Ke, Chien-Chih
Wu, Hsin-En
Chang, Hao-Han
Yeh, Hsin-Chih
Li, Chia-Yang |
| 貢獻者: | Kaohsiung Medical University
Kaohsiung Medical University Hospital
Chi Mei Hospital
Department of Senior Citizen Service Management, Chia Nan University of Pharmacy & Science
China Medical University Taiwan
Academia Sinica - Taiwan
National Health Research Institutes - Taiwan
Kaohsiung Municipal Ta-Tung Hospital |
| 關鍵字: | methyl protodioscin
dioscorea
invasion
injury
cycle |
| 日期: | 2022 |
| 上傳時間: | 2023-12-11 13:57:11 (UTC+8) |
| 出版者: | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER |
| 摘要: | Bladder cancer is one of the most common malignancies of the male genitourinary urinary system. Protodioscin is a steroidal saponin with anti-cancer effects on several types of cancers; however, the anti-cancer activities of protodioscin on bladder cancer have not yet been investigated. Therefore, we aimed to examine the anti-cancer effects of protodioscin on bladder cancer. Two types of bladder cancer cell lines, non-muscle-invasive 5637 cells and muscle-invasive T24 cells, were used to evaluate the effects of protodioscin on cell growth, migration, invasion and epithelial-mesenchymal transition(EMT) marker expressions. Transcriptome analysis was performed by RNA-seq and validated using real-time PCR and western blot; additionally, an in vivo xenograft animal model was established and the anti-tumor effects of protodioscin were tested. Our results demonstrated that protodioscin inhibited cell proliferation, migration, motility and invasion on 5637 and T24 cells. Additionally, protodioscin also induced cell apoptosis and arrested the progression of cell cycle at G2 phase in bladder cancer cells. Moreover, protodioscin inhibited EMT through increased protein expression of E-cadherin and decreased protein expression of N-cadherin and vimentin. RNA-seq analysis indicated that protodioscin regulated mitogenactivated protein kinase(MAPK) and phosphoinositide 3-kinases(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(mTOR) signaling pathways as further verified by Western blot. Furthermore, protodioscin |
| 關聯: | BIOMEDICINE & PHARMACOTHERAPY, v.156, December 2022, 113929 |
| 顯示於類別: | [高齡福祉養生管理系] 期刊論文
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