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    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34542


    標題: Characterization of CdSe QDs biosynthesized by a recombinant Rhodopseudomonas palustris
    作者: Jia, Qing-Yun
    Jia, Ru
    Chen, Chien-Min
    Wang, Lan
    貢獻者: Shanxi University
    Chia Nan University of Pharmacy & Science,Department of Environmental Resources Management
    Shanxi University
    關鍵字: cadmium-sulfide nanoparticles
    waste-water treatment
    quantum dots
    microbial synthesis
    extracellular biosynthesis
    strain rb
    toxicity
    cell
    copper
    metallothionein
    日期: 2023
    上傳時間: 2023-12-11 13:56:59 (UTC+8)
    出版者: ELSEVIER
    摘要: This study proposed a novel approach for the biosynthesis of CdSe quantum dots (QDs) by a recombinant photosynthetic bacteria Rhodopseudomonas palustris with metallothionein expression. The biosynthesis mecha-nisms and physico-chemical properties of the CdSe QDs were analyzed by various techniques, and its potential application was also investigated. The results showed that the biosynthesized CdSe QDs presented green fluorescence with maximum excitation and emission wavelengths at 460 and 530 nm, respectively. The quantum yield of the biosynthesized CdSe QDs was found out to be 3.85%. They were well-dispersed spherical particles with an average size of 5.37 +/- 0.06 nm. Their crystal structure was cubic with the d spacings of 3.1 angstrom and 2.5 angstrom, corresponded to the planes of (111) and (220), respectively. The biosynthesis process of CdSe QDs took place in the cytoplasm and may involve some proteins, peptides, lipids or other organic substances covering the surface of CdSe QDs through several specific functional groups, such as hydroxyl or sulfhydryl groups to maintain their stability. The purified CdSe QDs had good biocompatibility and great potential for cellular imaging application. It is concluded that the process may provide a more cost-effective and eco-friendly method not only to recover harmful cadmium in the environment but also to synthesize simultaneously a clean product for various industrial applications.
    關聯: Biochemical Engineering Journal, v.191, February 2023, 108771
    Appears in Collections:[休閒保健管理系(所)] 期刊論文

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