English  |  正體中文  |  简体中文  |  Items with full text/Total items : 18055/20253 (89%)
Visitors : 25232228      Online Users : 512
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34486


    標題: Biotransformation of celastrol to a novel, well-soluble, low-toxic and anti-oxidative celastrol-29-O-beta-glucoside by Bacillus glycosyltransferases
    作者: Chang, Te-Sheng
    Wang, Tzi-Yuan
    Chiang, Chien-Min
    Lin, Yu-Ju
    Chen, Hui-Lien
    Wu, Yu-Wei
    Ting, Huei-Ju
    Wu, Jiumn-Yih
    貢獻者: Natl Univ Tainan, Dept Biol Sci & Technol
    Acad Sinica, Biodivers Res Ctr
    Chia Nan Univ Pharm & Sci, Dept Biotechnol
    Taipei Med Univ, Coll Med Sci & Technol, Grad Inst Biomed Informat
    Taipei Med Univ Hosp, Clin Big Data Res Ctr
    Natl Quemoy Univ, Dept Food Sci
    關鍵字: Celastrol
    Glycosyltransferase
    Bacillus
    Triterpenoid
    Biotransformation
    日期: 2021
    上傳時間: 2023-11-11 11:56:52 (UTC+8)
    出版者: SOC BIOSCIENCE BIOENGINEERING JAPAN
    摘要: Celastrol is a quinone-methide triterpenoid isolated from the root extracts of Tripterygium wilfordii (Thunder god vine). Although celastrol possesses multiple bioactivities, the potent toxicity and rare solubility in water hinder its clinical application. Biotransformation of celastrol using either whole cells or purified enzymes to form less toxic and more soluble derivatives has been proven difficult due to its potent antibiotic and enzyme-conjugation property. The present study evaluated biotransformation of celastrol by four glycosyltransferases from Bacillus species and found one glycosyltransferase (BsGT110) from Bacillus subtilis with significant activity toward celastrol. The biotransformation metabolite was purified and identified as celastrol-29-O-beta-glucoside by mass and nuclear magnetic resonance spectroscopy. Celastrol-29-O-beta-glucoside showed over 53-fold higher water solubility than celastrol, while maintained 50% of the free radical scavenging activity of celastrol. When using zebrafish as the in vivo animal model, celastrol-29-O-beta-glucoside exhibited 50-fold less toxicity than celastrol. To our knowledge, the present study is not only the first report describing the biotransformation of celastrol, but also the first one detailing a new compound, celastrol-29-O-beta-glucoside, that is generated in the biotransformation process. Moreover, celastrol-29-O-beta-glucoside may serve as a potential candidate in the future medicine application due to its higher water solubility and lower toxicity. (C) 2020, The Society for Biotechnology, Japan. All rights reserved.
    關聯: J BIOSCI BIOENG, v.131, n.2, pp.176-182
    Appears in Collections:[生物科技系(所)] 期刊論文

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML146View/Open


    All items in CNU IR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback