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    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34477

    標題: Molecular characteristics and in vitro effects of antimicrobial combinations on planktonic and biofilm forms of Elizabethkingia anophelis
    作者: Tang, Hung-Jen
    Lin, Yi-Tsung
    Chen, Chi-Chung
    Chen, Chih-Wei
    Lu, Ying-Chen
    Ko, Wen-Chien
    Chen, Hung-Jui
    Su, Bo-An
    Chang, Ping-Chin
    Chuang, Yin-Ching
    Lai, Chih-Cheng
    貢獻者: Chi Mei Med Ctr, Dept Med
    Chi Mei Med Ctr, Dept Med Res
    Taipei Vet Gen Hosp, Dept Med, Div Infect Dis
    Natl Yang Ming Univ, Inst Emergency & Crit Care Med
    Natl Chiayi Univ, Dept Food Sci
    Chi Mei Med Ctr, Dept Surg, Div Neurosurg
    Chia Nan Univ Pharm & Sci, Coll Sustainable Environm, Dept Occupat Safety & Hlth, Inst Ind Safety & Disaster Prevent
    Natl Cheng Kung Univ Hosp, Dept Internal Med
    Natl Cheng Kung Univ, Coll Med, Dept Med
    Chi Mei Med Ctr, Dept Internal Med
    Kaohsiung Vet Gen Hosp, Dept Internal Med, Tainan Branch
    日期: 2021
    上傳時間: 2023-11-11 11:56:16 (UTC+8)
    摘要: Objectives: To investigate the in vitro activity of antibiotics against clinical Elizabethkingia anophelis isolates and to find a suitable antibiotic combination with synergistic effects to combat antibiotic-resistant E. anophelis and its associated biofilm. Methods: E. anophelis isolates were identified by 16S rRNA sequencing; 30 strains with different pulsotypes were identified and the MIC, antibiotic resistance mechanism, antibiotic combination activity and killing effects of antimicrobial agents on biofilms of these strains were determined. Results: All E. anophelis isolates were susceptible to minocycline and cefoperazone/sulbactam (1:1). More than 90% of clinical isolates were susceptible to cefoperazone/sulbactam(1:0.5), piperacillin/tazobactam and rifampicin. Some novel mutations, such as gyrA G81D, parE D585N and parC P134T, that have never been reported before, were identified. The synergistic effect was most prominent for the combination of minocycline and rifampicin, with 93.3% of their FIC index values <= 0.5, and no antagonism was observed using the chequerboard method. This synergistic effect between minocycline and rifampicin was also observed using time-killing methods for clinical E. anophelis isolates at both normal inoculum and high inoculum. Twenty-nine isolates tested positive for biofilm formation. Minocycline remained active against biofilm-embedded and biofilm-released planktonic E. anophelis cells; however, the enhanced effect of minocycline by adding rifampicin was only observed at 24 h (not at 72 and 120 h). Conclusions: Although E. anophelis was resistant to many antibiotics and could exhibit biofilm formation, minocycline showed potent in vitro activity against this pathogen and its associated biofilm.
    關聯: J ANTIMICROB CHEMOTH, v.76, n.5, pp.1205-1214
    Appears in Collections:[職業安全衛生系(含防災所)] 期刊論文

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