Zerumbone Suppresses the LPS-Induced Inflammatory Response and Represses Activation of the NLRP3 Inflammasome in Macrophages

doi:10.3389/fphar.2021.652860

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標題:	Zerumbone Suppresses the LPS-Induced Inflammatory Response and Represses Activation of the NLRP3 Inflammasome in Macrophages
作者:	Su, Chia-Cheng Wang, Shu-Chi Chen, I-Chen Chiu, Fang-Yen Liu, Po-Len Huang, Chi-Han Huang, Kuan-Hua Fang, Shih-Hua Cheng, Wei-Chung Huang, Shu-Pin Yeh, Hsin-Chih Liu, Ching-Chih Lee, Po-Yen Huang, Ming-Yii Li, Chia-Yang
貢獻者:	Kaohsiung Med Univ, Coll Med, Grad Inst Med Chi Mei Med Ctr, Div Urol, Dept Surg Chia Nan Univ Pharm & Sci, Dept Senior Citizen Serv Management Kaohsiung Med Univ, Dept Med Lab Sci & Biotechnol Kaohsiung Med Univ Hosp, Dept Pediat Kaohsiung Med Univ, Sch Med, Dept Pediat, Coll Med Kaohsiung Med Univ, Dept Resp Therapy, Coll Med Natl Taiwan Univ Sport, Inst Athlet China Med Univ, Grad Inst Biomed Sci, Res Ctr Canc Biol Kaohsiung Med Univ, Ctr Canc Res Kaohsiung Med Univ, Coll Med, Sch Med, Dept Urol Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Urol Kaohsiung Municipal Tatung Hosp, Dept Urol Chi Mei Med Ctr, Dept Ophthalmol, Taichung, Taiwan;[Lee, Po-Yen Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Ophthalmol Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Canc Ctr, Dept Radiat Oncol Kaohsiung Med Univ Hosp, Dept Med Res
關鍵字:	zerumbone macrophage inflammation NLRP3 inflammasome MAPKs
日期:	2021
上傳時間:	2023-11-11 11:51:57 (UTC+8)
出版者:	FRONTIERS MEDIA SA
摘要:	Zerumbone is a natural product isolated from the pinecone or shampoo ginger, Zingiber zerumbet (L.) Smith, which has a wide range of pharmacological activities, including anti-inflammatory effects. However, the effects of zerumbone on activation of the NLRP3 inflammasome in macrophages have not been examined. This study aimed to examine the effects of zerumbone on LPS-induced inflammatory responses and NLRP3 inflammasome activation using murine J774A.1 cells, murine peritoneal macrophages, and murine bone marrow-derived macrophages. Cells were treated with zerumbone following LPS or LPS/ATP treatment. Production of nitric oxide (NO) was measured by Griess reagent assay. The levels of IL-6, TNF-alpha, and IL-1 beta secretion were analyzed by ELISA. Western blotting analysis was performed to determine the expression of inducible NO synthase (iNOS), COX-2, MAPKs, and NLRP3 inflammasome-associated proteins. The activity of NF-kappa B was determined by a promoter reporter assay. The assembly of NLRP3 was examined by immunofluorescence staining and observed by confocal laser microscopy. Our experimental results indicated that zerumbone inhibited the production of NO, PGE(2) and IL-6, suppressed the expression of iNOS and COX-2, repressed the phosphorylation of ERK, and decreased the activity of NF-kappa B in LPS-activated J774A.1 cells. In addition, zerumbone suppressed the production of IL-1 beta and inhibited the activity of NLRP3 inflammasome in LPS/ATP- and LPS/nigericin-activated J774A.1 cells. On the other hand, we also found that zerumbone repressed the production of NO and proinflammatory cytokines in LPS-activated murine peritoneal macrophages and bone marrow-derived macrophages. In conclusion, our experimental results demonstrate that zerumbone effectively attenuates the LPS-induced inflammatory response in macrophages both in vitro and ex vivo by suppressing the activation of the ERK-MAPK and NF-kappa B signaling pathways as well as blocking the activation of the NLRP3 inflammasome. These results imply that zerumbone may be beneficial for treating sepsis and inflammasome-related diseases.
關聯:	FRONT PHARMACOL, v.12
显示于类别:	[高齡福祉養生管理系] 期刊論文

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