Cytotoxicity and cell imaging of six types of carbon nanodots prepared through carbonization and hydrothermal processing of natural plant materials

doi:10.1039/d1ra01318a

CNU IR > Pharmacy and Science > Dept. of Pharmacy > Periodical Articles > Item 310902800/34421

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34421

Title:	Cytotoxicity and cell imaging of six types of carbon nanodots prepared through carbonization and hydrothermal processing of natural plant materials
Authors:	Chen, Yu-Yu Jiang, Wen-Ping Chen, Huan-Luen Huang, Hui-Chi Huang, Guan-Jhong Chiang, Hsiu-Mei Chang, Chang-Cheng Huang, Cheng-Liang Juang, Tzong-Yuan
Contributors:	China Med Univ, Dept Cosmeceut Asia Univ, Dept Occupat Therapy Chia Nan Univ Pharm & Sci, Dept Pharm China Med Univ, Dept Chinese Pharmaceut Sci & Chinese Med Resourc China Med Univ, Master Program Food & Drug Safety China Med Univ Hosp, Aesthet Med Ctr China Med Univ, Sch Med Natl Chiayi Univ, Dept Appl Chem
Keywords:	GRAPHENE QUANTUM DOTS LABEL-FREE DETECTION GREEN SYNTHESIS ANTIINFLAMMATORY ACTIVITIES SILVER NANOPARTICLES MAHONIA-OIWAKENSIS FACILE SYNTHESIS DRUG-DELIVERY IN-VITRO NITROGEN
Date:	2021
Issue Date:	2023-11-11 11:51:31 (UTC+8)
Publisher:	ROYAL SOC CHEMISTRY
Abstract:	In this study we prepared six types of carbon nanodots (CNDs) from natural plant materials - through carbonization of two species of bamboo (Bamboo-I, Bamboo-II) and one type of wood (Wood), and through hydrothermal processing of the stem and root of the herb Mahonia oiwakensis Hayata (MO) and of the agricultural waste of two species of pineapple root (PA, PB). The resulting CNDs were spherical with dimensions on the nanoscale (3-7 nm); furthermore, CND-Bamboo I, CND-Wood, CND-Bamboo II, CND-MO, CND-PA, and CND-PB displayed fluorescence quantum yields of 9.63, 12.34, 0.90, 10.86, 0.35, and 0.71%, respectively. X-ray diffraction revealed that the carbon nanostructures possessed somewhat ordered and disordered lattices, as evidenced by broad signals at values of 2 theta between 20 and 30 degrees. CND-Bamboo I, CND-Wood, and CND-Bamboo II were obtained in yields of 2-3%; CND-MO, CND-PA, and CND-PB were obtained in yields of 17.64, 9.36, and 22.47%, respectively. Cytotoxicity assays for mouse macrophage RAW264.7 cells treated with the six types of CNDs and a commercial sample of Ag nanoparticles (NPs) revealed that each of our CNDs provided a cell viability of 90% at 2000 mu g mL(-1), whereas it was only 20% after treatment with the Ag NPs at 62.5 mu g mL(-1). The six types of CNDs also displayed low cytotoxicity toward human keratinocyte HacaT cells, human MCF-7 breast cancer cells, and HT-29 colon adenocarcinoma cells when treated at 500 mu g mL(-1). Moreover, confocal microscopic cell imaging revealed that the fluorescent CND-Bamboo I particles were located on the MCF-7 cell membrane and inside the cells after treatment for 6 and 24 h, respectively. We have thoroughly investigated the photoluminescence properties and carbon nanostructures of these highly dispersed CNDs. Because of the facile green synthesis of these six types of CNDs and their sourcing from abundant natural plants, herbs, and agriculture waste, these materials provide a cost-effective method, with low cytotoxicity and stable fluorescence, for biolabeling and for developing cell nanocarriers.
Relation:	RSC ADV, v.11, n.27, pp.16661-16674
Appears in Collections:	[Dept. of Pharmacy] Periodical Articles

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