LRWD1 expression is regulated through DNA methylation in human testicular embryonal carcinoma cells

doi:10.1186/s12610-021-00130-y

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標題:	LRWD1 expression is regulated through DNA methylation in human testicular embryonal carcinoma cells
作者:	Hung, Jui-Hsiang Cheng, Han-Yi Tsai, Yung-Chieh Pan, Hsien-An Omar, Hany A. Chiu, Chien-Chih Su, Yin-Mei Lin, Yung-Ming Teng, Yen-Ni
貢獻者:	Chia Nan Univ Pharm & Sci, Dept Biotechnol Natl Univ Tainan, Dept Biol Sci & Technol Chia Nan Univ Pharm & Sci, Chi Mei Med Ctr, Dept Obstet & Gynecol Chia Nan Univ Pharm & Sci, Dept Sport Management Chia Nan Univ Pharm & Sci, Dept Biotechnol An An Women & Children Clin Univ Sharjah, Sharjah Inst Med Res Univ Sharjah, Coll Pharm Beni Suef Univ, Fac Pharm, Dept Pharmacol Kaohsiung Med Univ, Dept Biotechnol Univ British Columbia, Fac Med, Dept Obstet & Gynecol
關鍵字:	LRWD1 DNA methylation 5-Aza-2 '-deoxycytidine Floxuridine
日期:	2021
上傳時間:	2023-11-11 11:51:29 (UTC+8)
出版者:	BMC
摘要:	Background: Sperm growth and maturation are correlated with the expression levels of Leucine-rich repeat and WD repeat-containing protein 1 (LRWD1), a widely expressed protein in the human testicles. The decrease in LRWD1 cellular level was linked to the reduction in cell growth and mitosis and the rise in cell microtubule atrophy rates. Since DNA methylation has a major regulatory role in gene expression, this study aimed at exploring the effect of the modulation of DNA methylation on LRWD1 expression levels. Results: The results revealed the presence of a CpG island up of 298 bps (- 253 similar to + 45) upon LRWD1 promoter in NT2/D1 cells. The hypermethylation of the LRWD1 promoter was linked to a reduction in the transcription activity in NT2/D1 cells, as indicated by luciferase reporter assay. The methylation activator, floxuridine, confirmed the decrease in the LRWD1 promoter transcriptional activity. On the other hand, 5-Aza-2'-deoxycytidine (5-Aza-dc, methylation inhibitor), significantly augmented LRWD1 promoter activity and the expression levels of mRNA and proteins. Furthermore, DNA methylation status of LRWD1 promoter in human sperm genomic DNA samples was analyzed. The results indicated that methylation of LRWD1 promoter was correlated to sperm activity. Conclusions: Thus, the regulation of LRWD1 expression is correlated with the methylation status of LRWD1 promoter, which played a significant role in the modulation of spermatogenesis, sperm motility, and vitality. Based on these results, the methylation status of LRWD1 promoter may serve as a novel molecular diagnostic marker or a therapeutic target in males' infertility.
關聯:	BASIC CLIN ANDROL, v.31, n.1
顯示於類別:	[生物科技系(所)] 期刊論文 [運動管理系] 期刊論文

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