Protective Effect of Piplartine against LPS-Induced Sepsis through Attenuating the MAPKs/NF-kappa B Signaling Pathway and NLRP3 Inflammasome Activation

doi:10.3390/ph14060588

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Title:	Protective Effect of Piplartine against LPS-Induced Sepsis through Attenuating the MAPKs/NF-kappa B Signaling Pathway and NLRP3 Inflammasome Activation
Authors:	Huang, Chi-Han Wang, Shu-Chi Chen, I-Chen Chen, Yi-Ting Liu, Po-Len Fang, Shih-Hua Huang, Shu-Pin Yeh, Hsin-Chih Liu, Ching-Chih Lee, Po-Yen Lin, Tzu-Chieh Cheng, Wei-Chung Su, Chia-Cheng Wu, Hsin-En Chen, Yuan-Ru Li, Chia-Yang
Contributors:	Kaohsiung Med Univ, Coll Med, Grad Inst Med Kaohsiung Med Univ, Dept Med Lab Sci & Biotechnol Kaohsiung Med Univ Hosp, Dept Pediat Kaohsiung Med Univ, Coll Med, Sch Med, Dept Pediat Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Pathol Kaohsiung Med Univ, Coll Med, Fac Med, Dept Pathol Kaohsiung Med Univ, Coll Med, Dept Resp Therapy Natl Taiwan Univ Sport, Inst Athlet Kaohsiung Med Univ, Coll Med, Sch Med, Dept Urol Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Urol Kaohsiung Municipal Tatung Hosp, Dept Urol Chi Mei Med Ctr, Dept Ophthalmol Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Ophthalmol Kaohsiung Med Univ, Dept Internal Med, Div Cardiol, Kaohsiung 80708, Taiwan;[Cheng, Wei-Chung China Med Univ, Res Ctr Canc Biol, Grad Inst Biomed Sci Chi Mei Med Ctr, Dept Surg, Div Urol Chia Nan Univ Pharm & Sci, Dept Senior Citizen Serv Management Kaohsiung Med Univ Hosp, Dept Med Res
Keywords:	piplartine macrophage inflammation NLRP3 inflammasome sepsis
Date:	2021
Issue Date:	2023-11-11 11:50:34 (UTC+8)
Publisher:	MDPI
Abstract:	Piplartine (or Piperlongumine) is a natural alkaloid isolated from Piper longum L., which has been proposed to exhibit various biological properties such as anti-inflammatory effects; however, the effect of piplartine on sepsis has not been examined. This study was performed to examine the anti-inflammatory activities of piplartine in vitro, ex vivo and in vivo using murine J774A.1 macrophage cell line, peritoneal macrophages, bone marrow-derived macrophages and an animal sepsis model. The results demonstrated that piplartine suppresses iNOS and COX-2 expression, reduces PGE(2), TNF-alpha and IL-6 production, decreases the phosphorylation of MAPKs and NF-kappa B and attenuates NF-kappa B activity by LPS-activated macrophages. Piplartine also inhibits IL-1 beta production and suppresses NLRP3 inflammasome activation by LPS/ATP- and LPS/nigericin-activated macrophages. Moreover, piplartine reduces the production of nitric oxide (NO) and TNF-alpha, IL-6 and IL-1 beta, decreases LPS-induced tissue damage, attenuates infiltration of inflammatory cells and enhances the survival rate. Collectively, these results demonstrate piplartine exhibits anti-inflammatory activities in LPS-induced inflammation and sepsis and suggest that piplartine might have benefits for sepsis treatment.
Relation:	PHARMACEUTICALS-BASE, v.14, n.6
Appears in Collections:	[Dept. of Senior Service and Health Management] Periodical Articles

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