Glycosylation of Ganoderic Acid G by Bacillus Glycosyltransferases

doi:10.3390/ijms22189744

CNU IR > Pharmacy and Science > Dept. of Cosmetic Science and institute of cosmetic science > Periodical Articles > Item 310902800/34373

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34373

Title:	Glycosylation of Ganoderic Acid G by Bacillus Glycosyltransferases
Authors:	Wu, Jiumn-Yih Ding, Hsiou-Yu Wang, Tzi-Yuan Zhang, Yun-Rong Chang, Te-Sheng
Contributors:	Natl Quemoy Univ, Dept Food Sci, Chia Nan Univ Pharm & Sci, Dept Cosmet Sci Acad Sinica, Biodivers Res Ctr Natl Univ Tainan, Dept Biol Sci & Technol
Keywords:	Bacillus Ganoderma lucidum glycosyltransferase saponin triterpenoid
Date:	2021
Issue Date:	2023-11-11 11:48:02 (UTC+8)
Publisher:	MDPI
Abstract:	Ganoderma lucidum is a medicinal fungus abundant in triterpenoids, its primary bioactive components. Although numerous Ganoderma triterpenoids have already been identified, rare Ganoderma triterpenoid saponins were recently discovered. To create novel Ganoderma saponins, ganoderic acid G (GAG) was selected for biotransformation using four Bacillus glycosyltransferases (GTs) including BtGT_16345 from the Bacillus thuringiensis GA A07 strain and three GTs (BsGT110, BsUGT398, and BsUGT489) from the Bacillus subtilis ATCC 6633 strain. The results showed that BsUGT489 catalyzed the glycosylation of GAG to GAG-3-o-beta-glucoside, while BsGT110 catalyzed the glycosylation of GAG to GAG-26-o-beta-glucoside, which showed 54-fold and 97-fold greater aqueous solubility than that of GAG, respectively. To our knowledge, these two GAG saponins are new compounds. The glycosylation specificity of the four Bacillus GTs highlights the possibility of novel Ganoderma triterpenoid saponin production in the future.
Relation:	INT J MOL SCI, v.22, n.18, pp.9744
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles

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