Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells

doi:10.1038/s41598-021-98005-9

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Title:	Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells
Authors:	Lee, Po-Yen Lai, Yu-Hung Liu, Po-Len Liu, Ching-Chih Su, Chia-Cheng Chiu, Fang-Yen Cheng, Wei-Chung Hsu, Shiuh-Liang Cheng, Kai-Chun Chiu, Li-Yi Kao, Tzu-En Lin, Chia-Ching Chang, Yo-Chen Wang, Shu-Chi Li, Chia-Yang
Contributors:	Kaohsiung Med Univ, Grad Inst Med, Coll Med Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Ophthalmol Kaohsiung Med Univ, Sch Med, Dept Ophthalmol, Coll Med Kaohsiung Med Univ, Dept Resp Therapy, Coll Med Chi Mei Med Ctr, Dept Ophthalmol Chi Mei Med Ctr, Div Urol, Dept Surg Chia Nan Univ Pharm & Sci, Dept Senior Citizen Serv Management China Med Univ, Grad Inst Biomed Sci China Med Univ, Res Ctr Tumor Med Sci China Med Univ, Drug Dev Ctr Kaohsiung Municipal Siaogang Hosp, Dept Ophthalmol Kaohsiung Municipal Tatung Hosp, Dept Ophthalmol Kaohsiung Med Univ, Dept Med Lab Sci & Biotechnol Kaohsiung Med Univ, Ctr Canc Res
Keywords:	EDEMA RIMANTADINE
Date:	2021
Issue Date:	2023-11-11 11:46:46 (UTC+8)
Publisher:	NATURE PORTFOLIO
Abstract:	Amantadine hydrochloride (HCl) is commonly prescribed for treating influenza A virus infection and Parkinson's disease. Recently, several studies have indicated that the use of amantadine HCl is associated with corneal edema; however, the cytotoxic effect of amantadine HCl has not been investigated. In the present study, the effects of amantadine HCl on cell growth, proliferation, and apoptosis in bovine cornea endothelial cells, and in vitro endothelial permeability were examined. Results showed that lower doses of amantadine HCl do not affect cell growth (<= 20 mu M), whereas higher doses of amantadine HCl inhibits cell growth (>= 50 mu M), induces apoptosis (2000 mu M), increases sub-G1 phase growth arrest (2000 mu M), causes DNA damage (>= 1000 mu M), and induces endothelial hyperpermeability (>= 1000 mu M) in bovine cornea endothelial cells; additionally, we also found that amantadine HCl attenuates the proliferation (>= 200 mu M) and arrests cell cycle at G1 phase (>= 200 mu M) in bovine cornea endothelial cells. In the present study, we measured the cytotoxic doses of amantadine HCl on cornea endothelial cells, which might be applied in evaluating the association of corneal edema.
Relation:	SCI REP-UK, v.11, n.1, pp.18514
Appears in Collections:	[高齡福祉養生管理系] 期刊論文

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