Gene signatures and potential therapeutic targets of Middle East respiratory syndrome coronavirus (MERS-CoV)-infected human lung adenocarcinoma epithelial cells

doi:10.1016/j.jmii.2021.03.007

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Title:	Gene signatures and potential therapeutic targets of Middle East respiratory syndrome coronavirus (MERS-CoV)-infected human lung adenocarcinoma epithelial cells
Authors:	Wu, Yen-Hung Yeh, I-Jeng Phan, Nam Nhut Yen, Meng-Chi Hung, Jui-Hsiang Chiao, Chung-Chieh Chen, Chien-Fu Sun, Zhengda Hsu, Hui-Ping Wang, Chih-Yang Lai, Ming-Derg
Contributors:	Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Emergency Med Kaohsiung Med Univ, Coll Med, Grad Inst Clin Med Nguyen Tat Thanh Univ, NTT Inst Hitechnol Chia Nan Univ Pharm & Sci, Dept Biotechnol I Shou Univ, Sch Chinese Med Postbaccalaureate Kaiser Permanente, Northern Calif Reg Labs, Permanente Med Grp Natl Cheng Kung Univ, Natl Cheng Kung Univ Hosp, Coll Med, Dept Surg Vanderbilt Univ, Dept Biostat, Med Ctr Taipei Med Univ, Coll Med Sci & Technol, PhD Program Canc Mol Biol & Drug Discovery Taipei Med Univ, Coll Med Sci & Technol, Grad Inst Canc Biol & Drug Discovery Natl Cheng Kung Univ, Dept Biochem & Mol Biol Natl Cheng Kung Univ, Coll Med, Inst Basic Med Sci
Keywords:	Coronavirus Middle East respiratory syndrome coronavirus (MERS-CoV) miRNA Bioinformatics Connectivity map Lung adenocarcinoma
Date:	2021
Issue Date:	2023-11-11 11:45:00 (UTC+8)
Publisher:	ELSEVIER TAIWAN
Abstract:	Background: Pathogenic coronaviruses include Middle East respiratory syndrome coronavirus (MERS-CoV), severe acute respiratory syndrome coronavirus (SARS-CoV), and SARS-CoV-2. These viruses have induced outbreaks worldwide, and there are currently no effective medications against them. Therefore, there is an urgent need to develop potential drugs against coronaviruses. Methods: High-throughput technology is widely used to explore differences in messenger (m) RNA and micro (mi)RNA expression profiles, especially to investigate proteineprotein interactions and search for new therapeutic compounds. We integrated miRNA and mRNA expression profiles in MERS-CoV-infected cells and compared them to mock-infected controls from public databases. Results: Through the bioinformatics analysis, there were 251 upregulated genes and eight highly differentiated miRNAs that overlapped in the two datasets. External validation verified that these genes had high expression in MERS-CoV-infected cells, including RC3H1, NF-kappa B, CD69, TNFAIP3, LEAP-2, DUSP10, CREB5, CXCL2, etc. We revealed that immune, olfactory or sensory system-related, and signal-transduction networks were discovered from upregulated mRNAs in MERS-CoV-infected cells. In total, 115 genes were predicted to be related to miRNAs, with the intersection of upregulated mRNAs and miRNA-targeting prediction genes such as TCF4, NR3C1, and POU2F2. Through the Connectivity Map (CMap) platform, we suggested potential compounds to use against MERS-CoV infection, including diethylcarbamazine, harpagoside, bumetanide, enalapril, and valproic acid. Conclusions: The present study illustrates the crucial roles of miRNA- mRNA interacting networks in MERS-CoV-infected cells. The genes we identified are potential targets for treating MERS-CoV infection; however, these could possibly be extended to other coronavirus infections. Copyright (C) 2021, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC.
Relation:	J MICROBIOL IMMUNOL, v.54, n.5, pp.845-857
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

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