Improving Aqueous Solubility of Natural Antioxidant Mangiferin through Glycosylation by Maltogenic Amylase from Parageobacillus galactosidasius DSM 18751

doi:10.3390/antiox10111817

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 化妝品應用與管理系(所) > 期刊論文 > Item 310902800/34344

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/34344

標題:	Improving Aqueous Solubility of Natural Antioxidant Mangiferin through Glycosylation by Maltogenic Amylase from Parageobacillus galactosidasius DSM 18751
作者:	Wu, Jiumn-Yih Ding, Hsiou-Yu Wang, Tzi-Yuan Tsai, Yu-Li Ting, Huei-Ju Chang, Te-Sheng
貢獻者:	Natl Quemoy Univ, Dept Food Sci Chia Nan Univ Pharm & Sci, Dept Cosmet Sci Acad Sinica, Biodivers Res Ctr Natl Univ Tainan, Dept Biol Sci & Technol
關鍵字:	mangiferin maltogenic amylase glycosylation glucoside Parageobacillus galactosidasius
日期:	2021
上傳時間:	2023-11-11 11:44:49 (UTC+8)
出版者:	MDPI
摘要:	Mangiferin is a natural antioxidant C-glucosidic xanthone originally isolated from the Mangifera indica (mango) plant. Mangiferin exhibits a wide range of pharmaceutical activities. However, mangiferin's poor solubility limits its applications. To resolve this limitation of mangiferin, enzymatic glycosylation of mangiferin to produce more soluble mangiferin glucosides was evaluated. Herein, the recombinant maltogenic amylase (MA; E.C. 3.2.1.133) from a thermophile Parageobacillus galactosidasius DSM 18751(T) (PgMA) was cloned into Escherichia coli BL21 (DE3) via the expression plasmid pET-Duet-1. The recombinant PgMA was purified via Ni2+ affinity chromatography. To evaluate its transglycosylation activity, 17 molecules, including mangiferin (as sugar acceptors), belonging to triterpenoids, saponins, flavonoids, and polyphenol glycosides, were assayed with beta -CD (as the sugar donor). The results showed that puerarin and mangiferin are suitable sugar acceptors in the transglycosylation reaction. The glycosylation products from mangiferin by PgMA were isolated using preparative high-performance liquid chromatography. Their chemical structures were glucosyl-alpha-(1 -> 6)-mangiferin and maltosyl-alpha-(1 -> 6)-mangiferin, determined by mass and nucleic magnetic resonance spectral analysis. The newly identified maltosyl-alpha-(1 -> 6)-mangiferin showed 5500-fold higher aqueous solubility than that of mangiferin, and both mangiferin glucosides exhibited similar 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activities compared to mangiferin. PgMA is the first MA with glycosylation activity toward mangiferin, meaning mangiferin glucosides have potential future applications.
關聯:	ANTIOXIDANTS-BASEL, v.10, n.11, pp.1817
显示于类别:	[化妝品應用與管理系(所)] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
antiox10111817.pdf		6981Kb	Adobe PDF	113	检视/开启
index.html		0Kb	HTML	189	检视/开启

在CNU IR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....