Adult inclusion conjunctivitis diagnosed by polymerase chain reaction and Giemsa stain

doi:10.1016/j.idcr.2021.e01367

CNU IR > Pharmacy and Science > Dept. of Cosmetic Science and institute of cosmetic science > Periodical Articles > Item 310902800/34310

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34310

Title:	Adult inclusion conjunctivitis diagnosed by polymerase chain reaction and Giemsa stain
Authors:	Lee, Wan-Ju Annabelle Chen, Chien-Chin
Contributors:	Chi Mei Med Ctr, Dept Ophthalmol Natl Cheng Kung Univ, Coll Med, Sch Pharm Natl Cheng Kung Univ, Coll Med, Inst Clin Pharm & Pharmaceut Sci Chung Hwa Univ Med Technol, Dept Optometry Chia Yi Christian Hosp, Dept Pathol, Ditmanson Med Fdn Chia Nan Univ Pharm & Sci, Dept Cosmet Sci
Keywords:	Adult inclusion conjunctivitis Chlamydial conjunctivitis PCR Giemsa stain Inclusion conjunctivitis Sexually transmitted disease STD
Date:	2021
Issue Date:	2023-11-11 11:42:34 (UTC+8)
Publisher:	ELSEVIER SCI LTD
Abstract:	Adult inclusion conjunctivitis, caused by Chlamydia trachomatis, is easily underdiagnosed with nonspecific ocular manifestation. Combined scrape cytology and molecular testing may be a useful strategy for its early diagnosis. A 24-year-old healthy male complained of blurred vision, foreign body sensation, and watery discharge in his right eye for four weeks. His visual acuity was 20/20 bilaterally at his first visit. Allergic conjunctivitis was the first impression, and topical treatment with corticosteroid and anti-histamine was prescribed. However, he returned five days later without symptom improvement, and his right eye vision declined to 20/40. Subepithelial corneal infiltration of his right eye was observed. According to his personal history, his girlfriend was diagnosed with sexually transmitted chlamydial infection and genital gonorrhea. Under the suspicion of sexually transmitted adult inclusion conjunctivitis, we collected his conjunctival lavage to both real-time polymerase chain reaction, which proved chlamydial infection, and Giemsa stain, which demonstrated typical basophilic intracytoplasmic inclusions. To diagnose adult inclusion conjunctivitis, we can use real-time polymerase chain reaction or Giemsa stain to help us obtain a quick and correct diagnosis. (C) 2021 The Authors. Published by Elsevier Ltd.
Relation:	IDCASES, v.27, e01367
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles

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