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    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34094


    標題: Cancer-Derived Transforming Growth Factor-beta Modulates Tumor-Associated Macrophages in Ampullary Cancer
    作者: Cheng, Li-Chin
    Chao, Ying-Jui
    Wang, Chih-Yang
    Phan, Nam Nhut
    Chen, Yi-Ling
    Wang, Tzu-Wen
    Hsu, Hui-Ping
    Lin, Yih-Jyh
    Shan, Yan-Shen
    Lai, Ming-Derg
    貢獻者: Chi Mei Med Ctr, Dept Surg, Div Colorectal Surg
    Natl Cheng Kung Univ, Natl Cheng Kung Univ Hosp, Coll Med, Dept Surg
    Natl Cheng Kung Univ, Coll Med, Inst Clin Med
    Natl Cheng Kung Univ, Coll Med, Dept Biochem & Mol Biol
    Natl Cheng Kung Univ, Coll Med, Inst Basic Med Sci
    Taipei Med Univ, Coll Med Sci & Technol, Program Canc Mol Biol & Drug Discovery
    Taipei Med Univ, Coll Med Sci & Technol, Grad Inst Canc Biol & Drug Discovery
    Nguyen Tat Thanh Univ, NTT Inst Hitechnol
    Chia Nan Univ Pharm & Sci, Senior Citizen Serv Management
    Vanderbilt Univ, Dept Biostat, Med Ctr, Nashville
    關鍵字: ampullary cancer
    tumor-associated macrophages
    transforming growth factor-beta
    bioinformatics
    日期: 2020
    上傳時間: 2022-11-18 11:23:41 (UTC+8)
    出版者: Dove Medical Press Ltd
    摘要: Purpose: Tumor-associated macrophages (TAMs) originate from monocytes and differentiate into mature macrophages. The interaction between cancer cells and TAMs promotes tumor growth and suppresses immunosurveillance. However, this phenomenon has seldom been observed in ampullary cancer. Patients and Methods: TAMs in ampullary cancer were investigated using immunohistochemical (IHC) staining of cancer tissues. Bioinformatic analysis of data from the Gene Expression Omnibus (GEO) database revealed transforming growth factor-beta (TGF-beta signaling in ampullary cancer. The complementary DNA microarray of cancer was compared with adjacent normal duodenum and enzyme-linked immunosorbent assay of serum was used to verify TGF-beta signaling in patients. The THP-1 cell line was activated in vitro to imitate M2 TAMs. ClueGo and CluePedia software were operated to simulate TGF-beta-related networks in ampullary cancer. Results: The IHC study revealed that the majority of TAMs inside ampullary cancer were cluster of differentiation (CD)163(+) cells and that the expression of mature CD68(+) macrophages was correlated with advanced cancer stage. Bioinformatics analysis revealed that TGF-beta and its downstream signaling were significantly upregulated. To verify our bioinformatics-derived predictions, we performed several experiments and demonstrated that increased TGF-beta expression was detected in the cDNA microarray. Higher serum levels of TGF-beta were correlated with fewer CD68(+) and more inducible nitric oxide synthase macrophages in ampullary cancer. Treatment with TGF-beta induced modulation of THP-1-derived macrophages. Conclusion: The present study demonstrates that TGF-beta modulates macrophage activity in ampullary cancer. Targeting TGF-beta could be an approach to activating immunosurveillance.
    關聯: Oncotargets and Therapy, v.13, pp.14
    Appears in Collections:[高齡福祉養生管理系] 期刊論文

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