Atractylodin ameliorates ovalbumin-induced asthma in a mouse model and exerts immunomodulatory effects on Th2 immunity and dendritic cell function

doi:10.3892/mmr.2020.11569

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 化妝品應用與管理系(所) > 期刊論文 > Item 310902800/34085

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標題:	Atractylodin ameliorates ovalbumin-induced asthma in a mouse model and exerts immunomodulatory effects on Th2 immunity and dendritic cell function
作者:	Lin, Yu-Chao Yang, Ching-Chieh Lin, Ching-Hsiung Hsia, Te-Chun Chao, Wen-Cheng Lin, Chi-Chien
貢獻者:	China Med Univ, Dept Med, Grad Inst Clin Med Sci China Med Univ, Dept Internal Med, Div Pulm & Crit Care Med China Med Univ, Dept Resp Therapy Chi Mei Hosp, Dept Radiat Oncol Natl Sun Yat Sen Univ, Inst Biomed Sci, Dept Sci Chia Nan Univ Pharm & Sci, Dept Pharm Changhua Christian Hosp, Div Chest Med, Dept Internal Med Taichung Vet Gen Hosp, Dept Crit Care Med Taichung Vet Gen Hosp, Dept Chest Med Tunghai Univ, Dept Ind Engn & Enterprise Informat Natl Chung Hsing Univ, Dept Life Sci, IEGG & Anim Biotechnol Ctr, Inst Biomed Sci China Med Univ Hosp, Dept Med Res
關鍵字:	asthma T helper 2 cells dendritic cells atractylodin Atractylodis rhizoma
日期:	2020
上傳時間:	2022-11-18 11:23:17 (UTC+8)
出版者:	Spandidos Publ Ltd
摘要:	Asthma is a leading allergic disease worldwide, demonstrating an ever-increasing prevalence over the past two decades. Asthma is characterized by allergen-associated airway hyperresponsiveness (AHR) that primarily results from T helper 2 (Th2) cell inflammation, in which dendritic cells (DCs) serve an important role in determining T cell development after encountering an antigen. Atractylodin (ATL), a polyethene alkyne extracted from Atractylodis rhizoma (also known as Cangzhu), has proven effective in treating digestive disorders, rheumatic disease and influenza. In addition, ATL was discovered to alleviate mouse collagen-induced arthritis via regulating DC maturation. The present study aimed to investigate the effect of ATL on asthma given that DCs serve an essential role in Th2-mediated inflammation in asthma. Mouse model of asthma was induced by ovalbumin (OVA). OVA-induced airway hyperresponsiveness (AHR) and inflammatory cells in bronchoalveolar lavage fluid (BALF) were detected. The production of IgE and IgG1 in serum and cytokines in BALF were detected by ELISA. The effects of ATL on dendritic cells maturation and T cell expansion were detected by flow cytometry analysis and 3H-thymidine incorporation. Using a model of OVA-induced asthma, it was demonstrated that ATL ameliorated AHR and decreased the levels of IL-4, IL-5 and IL-13 in bronchoalveolar lavage fluid (BALF), and OVA-specific IgE and IgG1 in the serum. OVA-stimulated splenocytes were used to demonstrated that ATL decreased cell expansion and the production of IL-4, IL-5 and IL-13 in the culture medium. In order to determine the cellular mechanism of ATL in asthma, splenic DCs were isolated and it was subsequently observed that ATL downregulated the expression levels of CD40 and CD80. Furthermore, OVA-stimulated CD4(+) T cells were co-cultured with splenic DCs, which revealed that ATL-treated splenic DCs led to impaired cellular proliferation and the production of IL-4, IL-5 and IL-13 in OVA-stimulated T cells. In conclusion, these results indicated that ATL may suppress antigen-specific Th2 responses in an OVA-induced allergic asthma model via regulating DCs. Therefore, ATL may exhibit therapeutic potential in the management of asthma and other allergic diseases presenting with Th2 inflammation.
關聯:	Molecular Medicine Reports, v.22, n.6, pp.10
顯示於類別:	[化妝品應用與管理系(所)] 期刊論文

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