Antiproliferative Effect and Mediation of Apoptosis in Human Hepatoma HepG2 Cells Induced by Djulis Husk and Its Bioactive Compounds

doi:10.3390/foods9101514

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Title:	Antiproliferative Effect and Mediation of Apoptosis in Human Hepatoma HepG2 Cells Induced by Djulis Husk and Its Bioactive Compounds
Authors:	Tu, Dom-Gene Chyau, Charng-Cherng Chen, Shih-Ying Chu, Heuy-Ling Wang, Shu-Chen Duh, Pin-Der
Contributors:	ChiaYi Christian Hosp, Ditmanson Med Fdn, Dept Nucl Med Natl Chung Cheng Univ, Dept Biomed Sci Hungkuang Univ, Biotechnol Res Inst Chia Nan Univ Pharm & Sci, Dept Hlth & Nutr Chia Nan Univ Pharm & Sci, Dept Food Sci & Technol
Keywords:	antiproliferation apoptosis djulis husk quercetin kaempferol
Date:	2020
Issue Date:	2022-11-18 11:22:56 (UTC+8)
Publisher:	Mdpi
Abstract:	The antiproliferative effect and mediation of apoptosis in human hepatoma HepG2 cells induced by djulis husk and its bioactive compounds was investigated. The ethanolic extracts of djulis husk (EEDH) at 50, 250, and 500 mu g/mL induced remarkable cytotoxicity on HepG2 cells. By flow cytometry analysis, EEDH slowed down the cell cycle at the Sub-G0 phase after 24 h of incubation. Moreover, all EEDH treatment induced an apoptotic response in HepG2 cells. EEDH-induced apoptosis was associated with the attenuation of mitochondrial transmembrane potentials (Delta psi(m)), an increase in Bax/Bcl-2 ratio, activation of caspase-3, and poly(ADP-ribose)polymerase (PARP) cleavage, as well as an increase in reactive oxygen species (ROS) generation. According to the HPLC-DAD and HPLC-MS/MS analysis, quercetin and kaempferol derivatives and another sixteen compounds were present in EEDH. Quercetin and kaempferol at 25-150 mu M showed antiproliferative action and induced apoptosis on HepG2 cells, which may in part account for the anticancer activity of EEDH. Overall, EEDH may be a potent chemopreventive agent due to apoptosis in HepG2 cells.
Relation:	Foods, v.9, n.10, pp.21
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] Periodical Articles [Dept. of Food Science & Technology] Periodical Articles

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