Anticancer activity and mediation of apoptosis in hepatoma carcinoma cells induced by djulis and its bioactive compounds

doi:10.1016/j.jff.2020.104225

CNU IR > Pharmacy and Science > Dept. of Health and Nutrition (including master's program) > Periodical Articles > Item 310902800/34074

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34074

Title:	Anticancer activity and mediation of apoptosis in hepatoma carcinoma cells induced by djulis and its bioactive compounds
Authors:	Chu, Chin-Chen Chen, Shih-Ying Chyau, Charng-Cherng Wu, You-Chia Chu, Heuy-Ling Duh, Pin -Der
Contributors:	Chi Mei Med Ctr, Dept Anesthesiol Chia Nan Univ Pharm & Sci, Dept Hlth & Nutr Hungkuang Univ, Res Inst Biotechnol Chia Nan Univ Pharm & Sci, Dept Food Sci & Technol
Keywords:	Anticancer activity Chemopreventive agent Djulis Hepatoma carcinoma cells Quercetin
Date:	2020
Issue Date:	2022-11-18 11:22:51 (UTC+8)
Publisher:	Elsevier
Abstract:	Anticancer activity and mediation of apoptosis in hepatoma carcinoma cells induced by ethanolic extracts of djulis (EECF) and its bioactive compounds were investigated. EECF (50-500 mu g/ml) exhibited significant cytotoxicity in HepG2 cells. AnnexinV-FITC/PI and DAPI staining assay showed that EECF induced apoptosis, accompanied with Sub-G0 phase arrest, ROS generation, loss of mitochondrial membrane potential (Delta Psi m), increase of Bax/Bcl-2 ratio, caspase-3 activation and PARP cleavage. According to the HPLC-DAD and HPLC-MS/ MS analysis, betanin, rutin, kaempferol, and quercetin were present in EECF, and showed antiproliferative action in HepG2 cells. Among the four bioactive compounds, quercetin showed the highest apoptotic effect toward HepG2 cells. In vivo, EECF and quercetin significantly reduced tumor growth in nude mice in tumor xenografts of HepG2 cells. Overall, the presence of bioactive compounds in EECF may account for the anticancer activity of EECF, and EECF could act as a potential chemopreventive agent against growth of hepatoma carcinoma cells.
Relation:	Journal of Functional Foods, v.75, pp.12
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] Periodical Articles [Dept. of Food Science & Technology] Periodical Articles

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