綠花白千層之成分分析及對痤瘡桿菌之抑制與抗發炎活性之研究

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標題:	綠花白千層之成分分析及對痤瘡桿菌之抑制與抗發炎活性之研究 Study on the compositions, anti-Cutibacterium acne and anti-inflammatory activites of Melaleuca quinquenervia essential oils 痤瘡為最常見的皮膚疾病之一，好發於面部、背部、手臂、胸部等等皮脂腺發達的部位，為了找出能有效控制痤瘡的物質，本實驗利用綠花白千層(Melaleuca quinquenervia) 的葉子，使用水蒸氣蒸餾法(SD)進行精油萃取，並進行成分分析及測定生物活性。利用氣相層析質譜分析儀(GC-MS)測定出萃取之綠花白千層精油之主成分為1,8-cineole 38.43%(醚類)、Azulene 17.21%(多環芳香烴)、D-Limonene 8.16%(單?類)、α-Pinene 7.70%(單?類)；市售綠花白千層精油主成分為1,8-cineole 51.55%(醚類)、Azulene 8.16%(多環芳香烴) 、α-Terpineol 7.70%(單?烯醇)、D-Limonene 6.69% (單?類)、α-Pinene 6.41%(單?類)，之後用這兩種精油測定其抗菌、抗發炎、脂肪?酵素抑制等活性分析，最後與抗生素進行協同作用的活性試驗，期望運用在化妝品上，增加其經濟價值。抗菌活性試驗結果顯示，綠花白千層精油對於痤瘡桿菌(Cutibacterium acne)是有顯著抑菌的效果，5-LOX脂氧合?抑制能力與NO自由基清除的測試顯示，在特定濃度下，綠花白千層精油具備抗發炎的能力，脂肪?酵素抑制試驗結果顯示，綠花白千層精油能有效抑制脂肪?酵素活性，最後在精油與抗生素的協同作用實驗中，顯示綠花白千層與抗生素是有協同作用抗菌能力。 Acne is one of the most common skin diseases. It occurs on the face, back, arms, chest and other areas with active sebaceous glands. In order to find an effective material to control acne, this experiment used steam distillation(SD) extraction method to extract Melaleuca quinquenervia essential oils and then analyze it composition and biological activity. The results showed that the main components of the extracted Melaleuca quinquenervia were 1,8-cineole 38.43% (ether), Azulene 17.21% (Polycyclic Aromatic Hydrocarbons), D-Limonene 8.16% (monoterpene), and α-Pinene 7.70%(monoterpene). The main components of commercially Melaleuca quinquenervia essential oil are 1,8-cineole 51.55% (ether) ,Azulene 8.16%(polycyclic aromatic hydrocarbons), α-Terpineol 7.70% (monoterpenol), D-Limonene 6.69% (monoterpene) , and α-Pinene 6.41%(monoterpene) by gas chromatography-mass spectrometry (GC-MS).Next, these two essential oils are determine their antibacterial, 5- lipoxygenase inhibition ability , NO free radical scavenging and lipase inhibition , we also conduct the synergistic activity test antibiotics, and time-kill curve assay of essential oils and antibiotic. The results of the antibacterial activity test showed that Melaleuca quinquenervia essential oil has a significant antibacterial effect on Cutibacterium acne. The inhibition of 5-lipoxygenase and NO free radical scavenging ability showed that Melaleuca quinquenervia essential oil has anti-inflammatory effects. Melaleuca quinquenervia essential oil can effectively inhibit the activity of lipase and have synergistic effect with antibiotic.
作者:	花翊恩
貢獻者:	化粧品應用與管理系蔡玫琳
關鍵字:	綠花白千層精油水蒸氣蒸餾法生物活性協同作用 Melaleuca quinquenervia Essential oils Steam distillation Bioactivity Synergistic effect
日期:	2020
上傳時間:	2022-10-21 10:31:47 (UTC+8)
關聯:	電子全文公開日期：2025-08-15 學年度：108， 83頁
顯示於類別:	[化妝品應用與管理系(所)] 博碩士論文

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