The aim of this study was to develop and assess nanostructured lipid carriers (NLC)compared to solid lipid nanoparticles (SLN) encapsulated with Astaxanthin. The purpose ofthe present research was to avoid oxidation and degradation of the active ingredient thenprolonged storage. In the this study, Encapsulation of Astaxanthin in SLN(Astaxnthin-SLN)and NLC(Astaxnthin-NLC) were prepared by high pressure homogenizers technique (500bars 5cycles). The experimental results show that SLN and NLC particle were around120nm-170nm with a narrow distribution and a stable zeta potential were around-25mV~-41mV. For all tested the total recovery of formulations were more than 90% and theencapsulation efficiency was 100%.We used Cetyl palmitate、Caprylic/Capric Triglycerideand Alkyl polyglucoside (APG) were prepared NLC ,that had better stability and lessdegradation than SLN. After storage for 3 months at 45℃, Astaxnthin-NLC were a moststable formulations that total recovery were more than 90% ,and by the skin irritation testresult was After storage for 3 months at 45℃,Astaxnthin-NLC were a most stableformulations that total recovery were more than 90% ,and by the skin irritation test result waslow irritation. The NLC has a characteristic of Occlusion effect, so we applied the serumcontaining NLC(S-NLC) on the face. The number of test 10 people and results showed that70% of people improved in moisture (P<0.05). The in vitro percutaneous absorption ofFranz cell results showed that Emulsion containing NLC(E-NLC) transdermal permeation ofAstaxanthin was greater than traditional-emulsion(T-emulsion), they were significantincreased (P < 0.05). These findings indicated that NLC could add to the traditional emulsionto improve the skin penetration.
