經聚乙烯亞胺處理後老鼠胚胎纖維母細胞微 RNA 表現之路徑分析

CNU IR > Pharmacy and Science > 2017 > Item 310902800/33572

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Title:	經聚乙烯亞胺處理後老鼠胚胎纖維母細胞微 RNA 表現之路徑分析
Authors:	郭榮華劉姮妤
Contributors:	藥學系
Date:	2017
Issue Date:	2022-01-25 14:44:48 (UTC+8)
Abstract:	Poly(ethylenimine) (PEI) is one of the most intensively used cationic polymers in non-viral nucleic acid delivery. Despite of great potentials of the applications in gene delivery systems, PEI has been shown to influence the regulations of gene expression in vitro and in vivo. Previous studies on PEI-induced gene regulation have primarily been based on encoding mRNAs that are translated into proteins. However, recent studies have demonstrated that non-coding RNAs are closely related to complex cellular development systems and various human diseases. Among these non-coding RNAs, microRNAs (miRNAs) are approximately 22 nucleotides long and primarily play important roles in the post-transcriptional regulation of gene expression, making them potential targets for therapeutic applications. Therefore, we explored the regulated miRNAs and identified their target genes in PEI-treated mouse embryonic fibroblast cells. The pathway analysis of target genes was performed using DIANA miRPath v.3.0, which is based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Our study may provide a deeper insight into the molecular pathways in cells caused by PEI.
Relation:	2017 第十一屆嘉南藥理大學藥理學院師生研究成果發表會，起迄日：2017/05/02-2017/05/04
Appears in Collections:	[Pharmacy and Science] 2017

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