本發明係一種多層微膠囊載體及其製備方法,尤其指一種由內至外包括一核心層、一維生素E層、一幾丁聚醣層,以及一油脂層之多層微膠囊載體及其製備方法,其中核心層包含一植物萃取物與一交聯結構的胜?;藉此,本發明之多層微膠囊載體透過將有效成分分層包覆,避免有效成分氧化或變質,且提供一種具有多功效之化妝保養品原料。 This invention relates to a multi-layered microcapsule and a manufacturing method thereof. The multi-layered microcapsule sequentially comprises a core layer, a vitamin E layer, a chitosan layer and an oil layer, wherein the core layer comprises a plant extract and a peptide with cross-linked structure. Thus, the multi-layered microcapsule of the present invention prevents oxidation of active ingredients effectively by coating them sequentially to provide a skin care material with multifunction.