製備鄰位-羥基異黃酮的方法

CNU IR > Pharmacy and Science > Dept. of Cosmetic Science and institute of cosmetic science > Patent > Item 310902800/33393

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Title:	製備鄰位-羥基異黃酮的方法 Method for Manufacturing Ortho-Hydroxyisoflavone
Authors:	丁秀玉張德生江建民
Contributors:	化粧品應用與管理系
Date:	2017-03-16
Issue Date:	2021-10-27 16:53:57 (UTC+8)
Abstract:	本發明係有關於一種製備鄰位-羥基異黃酮的方法，其包含下列步驟：製備一含有融合基因之環狀重組質體，其中融合基因係具有CYP57B3基因之跨膜區?(transmembrane?domain，TMD)、CYP105D7基因與細胞色素還原?sCPR (cytochrome P450 reductase)基因；以及將環狀重組質體置於一適合的微生物表達系統中，並利用含有異黃酮之培養基培養微生物表達系統一作用時間；藉此，可提高鄰位-羥基異黃酮 (ortho-hydroxyisoflavones)的產量。 The invention relates to a method for manufacturing an ortho-hydroxyisoflavone. It comprises the steps of preparing a cyclic recombinant plasmid including a fusion gene, wherein the fusion gene comprises a transmembrane?domain (TMD) of CYP57B3 gene, CYP105D7 gene, and sCPR (cytochrome P450 reductase) gene; transforming the cyclic recombinant plasmid into a suitable microbial expression system; and incubating the microbial expression system in a medium containing isoflavone therein for a period of time. Accordingly, the yield of the ortho-hydroxyisoflavone can be improved.
Relation:	專利編號：201710505 申請號：104130408 國際分類號：A61K-036/185(2006.01);A61P-029/00(2006.01) 專利公報：15卷06期
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Patent

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