In this study, Vigna angularis, Glycine max, Glycine max, Vigna radiata and Phaseolus coccineus were extracted by water immersion (BSE01~05) and ultrasonic extraction (BSE06~10). Ten extracts obtained from different extraction methods were analyzed by anti-MMP zymography for anti-aging investigation and disc diffusion method for anti-bacterial assay.
After the zymography analysis, the extracts, including BSE01, BSE05 and BSE07,were treated with 3T3 cell culture exhibited marked inhibitory capacities on MMP-2 and MMP-9 at the concentration of 5.0~10.0% in 48 h. The results from the anti-bacterial assay demonstrated that the extracts of BSE01~10 have no effects on the growth of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus.
To evaluate the synergistic activities of the above-mentioned three extracts solutions (BSE01, BSE05 and BSE07), the new three combined extracts (BSE11~13) were prepared by mixing with the equal amount of extracts (BSE01, BSE05 and BSE07) for anti-MMPs zymography. According to the zymography results, the most effective anti-aging extract, BSE11 (the mixture of BSE01 and BSE05) showed the significant suppression effect on MMP-2 and MMP-9 at the concentration of 5.0~10.0% in 24~48 h.
Finally, the BSE11 extract was added to make the skincare cream for its efficacy assessment on the subjects’ skin. The data from the efficacy testing demonstrated that the BSE11 cream applied to the 12 subjects’ faces for one month displayed obvious improvements on periorbital wrinkles of 10 subjects. Additionally, the BSE11 cream had no irritative effects on the subjects’ faces.
The above experimental results revealed that the BSE11 extract (the mixture of BSE01 and BSE05) possessed anti-MMP activity and anti-wrinkle ability, and it could be used for the development of anti-aging cosmetic ingredients.
