常綠喬木萃取物於美肌及抑制皮膚癌效能評估

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 化妝品應用與管理系(所) > 博碩士論文 > Item 310902800/33080

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標題:	常綠喬木萃取物於美肌及抑制皮膚癌效能評估 Evaluation of Evergreen Trees Extracts for Skin Beauty Bio-Activities and Skin Cancer Inhibition Effect
作者:	劉家綺
貢獻者:	化粧品應用與管理系梁家華
關鍵字:	美肌 skin beauty
日期:	2019
上傳時間:	2020-12-09 14:46:36 (UTC+8)
摘要:	本研究分別探討兩種水果第一部分以常見水果 DL為研究對象分開收集新鮮殼 (DL fresh hull, DLH F)與籽 (DL fresh seed, DLS F)及烘焙過的殼 (DL dried hull, DLH D)與籽 (DL dried seed , DLS D)利用乙醇和水萃取進行抗氧化、抗發炎、預防光老化及 DNA修復等試驗效能探討。無論是DL之乾品或鮮品皆具有清除 DPPH·和 ABTS· +能力，且 DLH D水萃物 (DLH DW)可以抑制人類皮膚角質細胞 (human skin immortalized keratinocyte, HaCaT)內活性氧物質(reactive oxygen species, ROS)及超氧陰離子 (superoxide, O2· 並提高穀胱甘肽之含量，同時透過增加超氧化物歧化酶 (superoxide dismutase, SOD)、穀胱甘肽過氧化物酶 (glutathione peroxidase, GPx)及過氧化氫酶 (catalase, CAT)而達到抗氧化之功效。另外， DLH DW可以透過抑制環氧合酶  ccyclooxygenase 2, COX 2)與一氧化氮合成酶 (nitric oxide synthases, iNOS)之基因表現進而降低腫瘤壞死因子、介白素 (interleukin 6, IL 6)、介白素之分泌。而在老化試驗中， DLH DW促進膠原蛋白 (collagen)生成及抑制玻尿酸酶分解並藉由提升修復因子的表現而預防因紫外線引起之光化產物嘧啶二聚體 (cyclobutane pyrimidine dimer, CPD)及二聚體 (6 4 pyrimidine pyrimidine photoproducts, 6 4PP)之生成。由本研究試驗結果得知，DL萃取物以 DLH DW抗氧化效果最佳，且同時具有抗發炎及預防光老化等效能。因此期許 DLH DW之抗皺與修復皮膚之作用未來可以應用於化妝品的活性成分。 The research was included two fruits. The first part was the common fruit- DL. The fresh hull (DLH-F), fresh seed (DLS-F), dried hull (DLH-D), and dried seed (DLS-D) were collected separately. The samples were extracted with ethanol and water and the effects of anti-oxidation, anti-inflammation, anti-photoaging and DNA repair were evaluated. DL extracts showed anti-oxidation effects by DPPH· and ABTS·+. DLH-D water extract (DLH-DW) could inhibit reactive oxygen species (ROS) and superoxide (O2·-) and increase glutathione (GSH) content and antioxidant enzymes in HaCaT cells. DLH-DW also inhibited tumor necrosis factor (TNF-a), interleukin IL-6, interleukin IL-1b by decreasing the gene expression of cyclooxygenase-2 (COX-2) and nitric oxide synthases (iNOS). Besides, DLH-DW could promote collagen production, degrade hyaluronidase, reduce the photochemical products cyclobutane pyrimidine dimer (CPDs) and 6-4 pyrimidine photoproducts (6-4PP) induced by UVB irradiation through regulating DNA repair factors. In this study, DLH-DW showed excellent anti-oxidation effect in DL extracts and it also had anti-inflammation and photoaging prevention effects. Therefore, DLH-DW may apply in cosmetic ingredient in the future.
關聯:	電子全文校內公開日期：2024-07-31;校外：2024-09-02 學年度：107，100頁
显示于类别:	[化妝品應用與管理系(所)] 博碩士論文

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