絲狀真菌能夠產生不同結構和多種生物活性之二次代謝物，廣泛應用於醫藥、生技和食品等領域。費氏麴菌 (Neosartorya fischeri) 為嗜熱性菌，通常生長於潮濕環境中，如土壤、加工食品和腐爛植被等，主要二次代謝物為乙醯基環帶麴菌素 (acetylaszonalenin)。本實驗室先前研究中發現，乙醯基環帶麴菌素對其他細胞株具有細胞毒性，但是相同濃度下對人類角質形成細胞卻不會產生細胞毒性。為確認此化合物在化粧品領域潛在的生物活性，本研究自費氏麴菌二次代謝物中純化出乙醯基環帶麴菌素，並以永生性人類HaCaT 角質細胞做為模式細胞株，使用基因微陣列技術分析經由乙醯基環帶麴菌素處理後之細胞中具有顯著變化的基因，再進行基因富集分析歸納出這些基因所涉及之路徑。結果發現乙醯基環帶麴菌素可能影響HaCaT 角質細胞中的脂質代謝、細胞生長和分化。後續透過即時定量聚合酶鏈鎖反應，結果顯示參與其生物合成路徑中之基因表現降低，推測可能造成生物合成下降以維持皮膚屏障中各項成分比例間的平衡，希望乙醯基環帶麴菌素未來能夠開發為新成分應用於化粧品產業或醫藥領域。 Filamentous fungi produce secondary metabolites with distinct structures and
various biological activities for a wide range in medical, biotechnology, and food fields. Neosartorya fischeri is a heat-resistant fungus usually grown in a humid environment such as soil, processed food, and decaying vegetation.
Acetylaszonalenin is the major secondary metabolite in N. fischeri, but there are few kinds of literature and studies on the bioactivity of acetylaszonalenin. In our previous study, we found that acetylaszonalenin showed high cytotoxicity in human adenocarcinoma cells but low cytotoxicity in human keratinocytes. For elucidating the potential and possible bioactivity of this compound in cosmetic field, human keratinocytes were used as experimental cell lines. To better
understand the mechanisms involved in the response of human keratinocytes to
acetylaszonalenin, microarray analysis was used to help identify significantly
expressed genes that may contribute to the response. After obtaining a largescale survey of the variations in gene expression patterns and gene ontology enrichment analysis, we found that acetylaszonalenin might affect the lipid metabolism, cell growth and differentiation of keratinocytes. The expression of candidate genes involved in these pathways were confirmed by real-time quantitative polymerase chain reaction analysis. In future, we hope that acetylaszonalenin could be applied to develop the ingredients in cosmetic or medical field.
