| 摘要: | 肝細胞癌 (hepatocellular carcinoma , HCC) 是最常見的肝癌型式，由肥胖、糖尿病、非酒精性脂肪肝 (nonalcoholic fatty liver disease, NAFLD) 所引起。本研究目的是探討50%乙醇紅藜萃取物 (50% ethanolic extracts from Chenopodium formosanum, EECF)，及其活性成分Rutin, Quercetin, Kaempferol和 Betanin 對HepG2細胞增殖與凋亡的影響。結果顯示 EECF, Quercetin及Kaempferol具有顯著抑制HepG2增殖的效應；EECF (500-750 μg/ml) 可藉由增加乳酸脫氫酶 (lactate dehydrogenase release, LDH) 釋放、提高細胞內氧化壓力、調降粒線體膜電位與活化caspase-3活性，最終導致HepG2細胞凋亡。此外，Quercetin (200 μM) 與Kaempferol (100-200 μM) 亦具有與 EECF 相同的細胞凋亡之作用機制，惟無法提升細胞內氧化壓力。再者，將 HepG2 細胞異種移殖在BALA/c (nu/nu) 裸鼠後，經管餵 EECF (100 mg/kg bw) 或Quercetin (20-40 mg/kg bw) 21 天，其腫瘤體積顯著降低。推測EECF具有抗增殖作用的原因，可能與其中含有Quercetin及Kaempferol等成分有關。總結，由上述 in vitro與in vivo試驗結果，EECF具有在肝癌治療上之潛在應用參考價值。
The burden of hepatocellular carcinoma (HCC), the most common form of liver cancer, is steadily growing because obesity, diabetes, nonalcoholic fatty liver disease (NAFLD) and viral-related liver disease. The aim of this study was to investigate the effects of 50% ethanolic extracts from Chenopodium formosanum (EECF) including rutin, quercetin, kaempferol and betanin on HepG2 cells proliferation or apoptosis. Results showed that EECF, quercetin and kaempferol inhibit HepG2 proliferation, significantly. EECF (500-750 μg/ml) induces apoptosis of HepG2 cells through an increase in the lactate dehydrogenase release (LDH), the downregulation of mitochondrial membrane potential and the up-regulation of caspase-3 and excessive intracellular ROS generation. Quercetin (200 μM) and kaempferol (100-200 μM) induces apoptosis of HepG2 cells through the same as the mechanism of EECF, except for intracellular ROS generation. Besides, when HepG2 cells are grown as xenografts in BALA/c (nu/nu) nude mice, the oral administration of EECF (100 mg/kg bw) or quercetin (20-40 mg/kg bw) for 21 days induce a significant decrease in tumor volume. According to the research, quercetin and kaempferol were present in EECF, which may account for its antiproliferative effect. Collectively, from the above in vitro and in vivo results; EECF could be a potential reference for the therapeutic applications of liver cancer. |
