English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 18034/20233 (89%)
造訪人次 : 23356773      線上人數 : 454
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/32972


    標題: 蛹蟲草萃出物對DEHP誘導的血管平滑肌細胞遷移及MMP-2和MMP-9表現的預防作用
    Preventive Effects of Cordyceps militaris Extract on Vascular Smooth Muscle Cells Migration and Expressions of MMP-2 and MMP-9 in Responding to DEHP Induction
    作者: 董育振
    貢獻者: 藥學系
    施美份
    關鍵字: 蛹蟲草
    MMP-2
    MMP-9
    動脈粥狀硬化
    Cordyceps militaris
    MMP-2
    MMP-9
    Atherosclerosis
    日期: 2019
    上傳時間: 2020-12-09 14:42:27 (UTC+8)
    摘要: 血管平滑肌過度表現MMP-2 及 MMP-9蛋白質以及平滑肌細胞的轉移被認為與動脈粥樣化的病程發展有密切關聯。MMP-2 及 MMP-9的蛋白質由許多的調節路徑所調控,如nuclear factor kappa (NF-κB),p38 mitogen activated protein kinase (MAPK),extracellular signal regulated kinase 1 & 2 (ERK 1/2),及 Akt。Di(2-ethylhexyl) phthalate (DEHP)是常見的一種塑化劑,近年來被發現與動脈粥樣化的發生或發展有相關性,其機轉可能包括增加血管平滑肌的MMP-2及MMP-9蛋白質表現有關。研究發現蛹蟲草萃出物可以預防高血脂症,然而蛹蟲草萃出物對於DEHP-誘發動脈粥樣化的保護機制從未被揭露過。血管平滑肌以5 ppm DEHP及蛹蟲草萃出物(濃度由1-50 μg/ml)作用24小時之後檢視MMP-2及MMP-9蛋白質表現、作用20分鐘之後檢視p38 MAPK、Akt、ERK1/2蛋白質表現、以及12小時之後檢視NF-κB蛋白質表現。結果發現:蛹蟲草萃出物可以有效抑制DEHP 5 ppm刺激的MMP-2及MMP-9蛋白質表現,且為一種濃度依賴性的關係。此外,DEHP-誘導的p38 MAPK、ERK1/2、Akt、以及NF-κB蛋白質表現以及血管平滑肌遷移也受到蛹蟲草萃出物的抑制。總結,蛹蟲草萃出物藉由抑制MMP-2及MMP-9蛋白質表現的調節路徑分子(p38 MAPK、ERK1/2、Akt、以及NF-κB)表現,具有潛在發展成為抑制動脈粥樣化的病程惡化的藥物。
    Over-expressions of MMP-2 and MMP-9 and cell migration of vascular smooth muscle cells (VSMC) have been considered as two of several biomarkers in the progression of atherosclerosis. Several signal transduction systems have been linked to control expressions of MMP-2 and MMP-9, among them are nuclear factor kappa (NF-κB), p38 mitogen activated protein kinase (MAPK), extracellular signal regulated kinase 1 & 2 (ERK 1/2), and Akt. Atherosclerosis can be induced by di(2-ethylhexyl) phthalate (DEHP), which is one of the most common compounds used as plasticizers in most of plastic products. The possible mechanisms of DEHP-induced atherosclerosis include increasing MMP-2 and MMP-9 expression and these regulatory pathways in VSMC. Cordyceps militaris extract has been shown its capability to prevent hyperlipidemia. However, its protective mechanisms against DEHP-induced expressions of MMP-2 and MMP-9 were not elucidated previously. vascular smooth muscle cells (VSMC) was treated with 5 ppm DEHP with or without Cordyceps militaris extract (dose between 1-50 μg/ml) for 24h prior to MMP-2 and MMP-9, 20 min prior to AMPK, ERK1/2, and Akt, and 12h prior to NF-kB expressions. DEHP-induced MMP-2 and MMP-9 expressions were prevented by treatment of Cordyceps militaris extract in a dose dependent manner. In addition, DEHP-induced phosphorylated MAPK, ERK1/2, Akt, and NF-κB expressions and VSMC migration were also inhibited by Cordyceps militaris extract treatment. In conclusion, Cordyceps militaris extract may have potential role in preventing DEHP-induced atherosclerosis progression by inhibiting MMP-2 and MMP-9 expressions via MAPK, ERK1/2, Art, and NF-κB pathways.
    關聯: 電子全文公開日期:2024-08-15
    學年度:107,56頁
    顯示於類別:[藥學系(所)] 博碩士論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    index.html0KbHTML831檢視/開啟


    在CNU IR中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋