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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/32854


    標題: 製備鄰位-羥基異黃酮的方法
    Method for Manufacturing Ortho-Hydroxyisoflavone
    作者: 丁秀玉
    張德生
    江建民
    貢獻者: 化粧品應用與管理系
    生物科技系
    日期: 2015-09-15
    上傳時間: 2020-12-01 14:16:46 (UTC+8)
    摘要: 本發明係有關於一種製備鄰位-羥基異黃酮的方法,其包含下列步驟:製備一含有融合基因之環狀重組質體,其中融合基因係具有CYP57B3基因之跨膜區?(transmembrane?domain,TMD)、CYP105D7基因與細胞色素還原?sCPR (cytochrome P450 reductase)基因;以及將環狀重組質體置於一適合的微生物表達系統中,並利用含有異黃酮之培養基培養微生物表達系統一作用時間;藉此,可提高鄰位-羥基異黃酮 (ortho-hydroxyisoflavones)的產量。 The invention relates to a method for manufacturing an ortho-hydroxyisoflavone. It comprises the steps of preparing a cyclic recombinant plasmid including a fusion gene, wherein the fusion gene comprises a transmembrane?domain (TMD) of CYP57B3 gene, CYP105D7 gene, and sCPR (cytochrome P450 reductase) gene; transforming the cyclic recombinant plasmid into a suitable microbial expression system; and incubating the microbial expression system in a medium containing isoflavone therein for a period of time. Accordingly, the yield of the ortho-hydroxyisoflavone can be improved.
    關聯: 專利編號:I545198;公開號:201710505
    申請號:104130408
    國際分類號:C12P-017/06(2006.01);C12N-015/81(2006.01);C07D-311/36(2006.01);C12R-001/84(2006.01)
    專利公報:43卷23期
    Appears in Collections:[化妝品應用與管理系(所)] 專利
    [生物科技系(所)] 專利

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