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| 標題: | The effects of human parvovirus VP1 unique region in a mouse model of allergic asthma |
| 作者: | Shyh-Ren Chiang(蔣士仁)
Lin, Chia-Yun
Chen, Der-Yuan
Tsai, Hui-Fang
Lin, Xin-Ci
Hsu, Tsai-Ching
Tzang, Bor-Show |
| 貢獻者: | Chi Mei Med Ctr, Dept Internal Med
Chia Nan Univ Pharm & Sci, Dept Gen Sci
Chung Shan Med Univ, Inst Biochem Microbiol & Immunol
China Med Univ Hosp, Rheumatol & Immunol Ctr
China Med Univ Hosp, Dept Med Res, Rheumat Dis Res Ctr
China Med Univ, Sch Med
Chung Shan Med Univ, Dept Med Lab & Biotechnol
Chung Shan Med Univ Hosp, Clin Lab
Chung Shan Med Univ, Immunol Res Ctr
Chung Shan Med Univ, Sch Med, Dept Biochem |
| 關鍵字: | RESPIRATORY-TRACT INFECTION
HUMAN BOCAVIRUS
B19 INFECTION
IGE
EXACERBATION
VIRUSES
PROTEIN
HYPERREACTIVITY
ASSOCIATION
INDUCTION |
| 日期: | 2019-05 |
| 上傳時間: | 2020-07-29 13:55:09 (UTC+8) |
| 出版者: | PUBLIC LIBRARY SCIENCE |
| 摘要: | Evidence has indicated that viral infection increases the risk of developing asthma. Although the association of human parvovirus B19 (B19V) or human bocavirus (HBoV) with respiratory diseases has been reported, little is known about the influence of the B19V-VP1u and HBoV-VP1u proteins on the symptoms of asthma. Herein, we investigated the systemic influence of subcutaneously injected B19V-VP1u and HBoV-VP1u recombinant proteins in an OVA-sensitized asthmatic mouse model. A significantly higher Penh ratio and IgE level were detected in the serum, bronchoalveolar lavage fluid (BALF) and the supernatant of a lymphocyte culture from mice treated with HBoV-VP1u or B19V-VP1u than in a lymphocyte culture from OVA-sensitized mice. Significantly higher levels of serum and BALF IgE, total IgG, IgG1, OVA-specific IgE and OVA-specific IgG1 were detected in mice treated with HBoV-VP1u or B19V-VP1u than in OVA-sensitized mice. Conversely, a significantly lower IgG2a level was detected in mice from the HBoV-VP1u or B19V-VP1u groups than in mice from the OVA group. The mice treated with HBoV-VP1u or B19V-VP1u exhibited more significant lung inflammatory indices, including elevated serum and BALF IL-4, IL-5, IL-10 and IL-13 levels; BALF lymphocyte, neutrophil and eosinophil counts, MMP-9 and MMP-2 activity; and the amount of lymphocyte infiltration, relative to those in the control mice or in those sensitized with OVA. These findings demonstrate that the subcutaneous injection of HBoV-VP1u or B19V-VP1u proteins in OVA-sensitized mice result in elevated asthmatic indices and suggest that human parvoviruses may increase the risk of developing airway inflammation in a mouse model of asthma. |
| 關聯: | Plos One, v.14, n.5, e0216799 |
| 顯示於類別: | [通識教育中心] 期刊論文
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