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| 標題: | Tris(8-Hydroxyquinoline)iron induces apoptotic cell death via oxidative stress and by activating death receptor signaling pathway in human head and neck carcinoma cells |
| 作者: | Chan, Leong-Perng
Tseng, Ya-Ping
Hsiou-Yu Ding(丁秀玉)
Pan, Sheng-Ming
Chiang, Feng-Yu
Wang, Ling-Feng
Chou, Tzung-Han
Lien, Pei-Jung
Liu, Cheng
Kuo, Po-Lin
Chia-Hua Liang(梁家華) |
| 貢獻者: | Kaohsiung Med Univ, Kaohsiung Municipal Ta Tung Hosp, Dept Otorhinolaryngol Head & Neck Surg, Kaohsiung, Taiwan
Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Coll Med, Fac Med
Kaohsiung Med Univ, Grad Inst Clin Med
Natl Cheng Kung Univ, Inst Basic Med Sci
Chia Nan Univ Pharm & Sci, Dept Cosmet Sci
Chia Nan Univ Pharm & Sci, Inst Cosmet Sci
Nat Chung Shan Inst Sci & Technol, Chem Syst Res Div Propellant Plant
Natl Yunlin Univ Sci & Technol, Dept Chem & Mat Engn, Touliu
Met Ind Res & Dev Ctr
Shu Zen Jr Coll Med & Managemen4, Dept Dent Technol
Natl Sun Yat Sen Univ, Inst Med Sci & Technol |
| 關鍵字: | Tris(8-Hydroxyquinoline)iron
Human head and neck carcinoma
Oxidative stress
Mitochondria
Death receptor |
| 日期: | 2019-10 |
| 上傳時間: | 2020-07-29 13:54:01 (UTC+8) |
| 出版者: | ELSEVIER GMBH |
| 摘要: | Background: 8-Hydroxyquinoline derivatives have highly sensitive fluorescent chemosensors for metal ions, which are associated with anti-oxidant, anti-tumor and anti-HIV-1 properties. Head and neck squamous cell carcinoma (HNSCC) is associated with a high rate of mortality and novel anti-HNSCC drugs must be developed. Therefore, effective chemotherapy agents are required to address this public health issue. Hypothesis/Purpose: The aim of this study was to investigate the inhibitory effect of tris(8-hydroxyquinoline)iron (Feq(3)) on the HNSCC and the underlying mechanism. Study design/Methods: A novel 8-hydroxyquinoline derivative, Feq(3), was synthesized. The cell viabilities were analyzed using MTT reagent. Apoptosis and the cell cycle distributions were determined by flow cytometer. Reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescence, western blot, MitoSOX and CellROX stain assay were used to study the mechanism of Feq(3). Feq(3) combined with antioxidants NAC (N-acetylcysteine) and BSO (buthionine sulfoximine) measured the cell viability and intracellular ROS. Results: Feq(3) induced the death of HNSCC cells and caused them to exhibit the morphological features of apoptosis. Feq(3) also induced apoptosis of SCC9 cells by cell cycle arrest during the G(2)/M phase and the induced arrest of SCC25 cells in the G(0)/G(1) and G(2)/M phases, which was associated with decreased cyclin B1/cdc2 and cyclin D/cdk4 expressions. Feq(3) increases reactive oxygen species (ROS) and reduces glutathione (GSH) levels, and responds to increased p53 and p21 expressions. Feq(3) induced apoptosis by mitochondria-mediated Box and cytochrome c up-expression and down-expression Bcl-2. Feq(3) also up-regulated tBid, which interacts with the mitochondrial pathway and tumor necrosis factor-alpha (TNF-alpha)/TNF-Rs, FasL/Fas, and TNF-related apoptosis inducing ligand receptors (TRAIL-Rs)/TRAIL-dependent caspases apoptotic signaling pathway in HNSCC cells. However, Feq(3) activates Fas but not FasL in SCC25 cells. Feq(3) arrests the growth of HNSCC cells and is involved in the mitochondria- and death receptor (DR)-mediated caspases apoptotic pathway. Conclusion: This study is the first to suggest that apoptosis mediates the anti-HNSCC of Feq(3). Feq(3) has potential as a cancer therapeutic agent against HNSCC. |
| 關聯: | Phytomedicine, v.63, 153005 |
| 顯示於類別: | [化妝品應用與管理系(所)] 期刊論文
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