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    Title: Molecular damage and responses of oral keratinocyte to hydrogen peroxide
    Authors: Lin, Kuan-Yu
    Chung, Ching-Hung
    Ciou, Jheng-Sian
    Su, Pei-Fang
    Wang, Pei-Wen
    Shieh, Dar-Bin
    Tzu-Chueh Wang(王四切)
    Contributors: Penn State Univ, Dept Biochem & Mol Biol, Harrisburg
    Natl Cheng Kung Univ Hosp, Dept Stomatol
    Chia Nan Univ Pharm & Sci, Grad Inst Pharmaceut Sci
    Natl Cheng Kung Univ, Dept Stat, Tainan 70101, Taiwan
    Natl Cheng Kung Univ, Natl Cheng Kung Univ Hosp, Coll Med, Inst Oral Med
    Natl Cheng Kung Univ, Natl Cheng Kung Univ Hosp, Coll Med, Dept Stomatol
    Natl Cheng Kung Univ, Innovat Ctr Adv Med Device Technol, Adv Optron Technol Ctr, Ctr Appl Nanomed,Ctr Micro Nano Sci & Technol
    Chia Nan Univ Pharm & Sci, Dept Pharm
    Keywords: Tooth bleaching
    Molecular genetics
    Oxidative stress
    Date: 2019-01
    Issue Date: 2020-07-29 13:53:58 (UTC+8)
    Publisher: BMC
    Abstract: BackgroundHydrogen peroxide (H2O2)-based tooth bleaching reagents have recently increased in popularity and controversy. H2O2 gel (3%) is used in a Nightguard for vital bleaching; transient tooth sensitivity and oral mucosa irritation have been reported. Genotoxicity and carcinogenicity have also been significant concerns.MethodsWe used primary cultured normal human oral keratinocytes (NHOKs) as an in vitro model to investigate the pathological effects to mitochondria functions on human oral keratinocytes exposed to different doses of H2O2 for different durations.ResultsAn MTT assay showed compromised cell viability at a dose over 5mM. The treatments induced nuclear DNA damage, measured using a single-cell gel electrophoresis assay. A real-time quantitative polymerase chain reaction showed H2O2 induced significant increase in mitochondrial 4977-bp deletion. Mitochondrial membrane potential and apoptosis assays suggested that oxidative damage defense mechanisms were activated after prolonged exposure to H2O2. Reduced intracellular glutathione was an effective defense against oxidative damage from 5mM of H2O2.ConclusionOur study suggests the importance for keratinocyte damage of the dose and the duration of the exposure to H2O2 in at-home-bleaching. A treatment dose 100mM directly causes severe cytotoxicity with as little as 15min of exposure.
    Relation: Bmc Oral Health, v.19, 10
    Appears in Collections:[Dept. of Pharmacy] Periodical Articles

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