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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/32655

    標題: A thrombomodulin-like gene is crucial to the collective migration of epibolic blastomeres during germ layer formation and organogenesis in zebrafish
    作者: Lee, Gang-Hui
    Chang, Chia-Lin
    Chiu, Wen-Tai
    Hsiao, Tsun-Hsien
    Chen, Po-Yuan
    Kuan-Chieh Wang(王冠傑)
    Kuo, Cheng-Hsiang
    Chen, Bing-Hung
    Shi, Guey-Yueh
    Wu, Hua-Lin
    Fu, Tzu-Fun
    貢獻者: Natl Cheng Kung Univ, Coll Med, Inst Basic Med Sci
    Natl Cheng Kung Univ, Int Ctr Wound Repair & Regenerat
    Natl Cheng Kung Univ, Coll Med, Dept Biochem & Mol Biol
    Natl Cheng Kung Univ, Coll Med, Cardiovasc Res Ctr
    Natl Cheng Kung Univ, Coll Engn, Dept Biomed Engn, Tainan, Taiwan
    Natl Cheng Kung Univ, Coll Med, Dept Med Lab Sci & Biotechnol
    Natl Cheng Kung Univ, Coll Med, Dept Food Safety Hyg & Risk Management
    Chia Nan Univ Pharm & Sci, Coll Pharm & Sci, Dept Pharm
    Kaohsiung Med Univ, Dept Biotechnol
    Natl Sun Yat Sen Univ, Inst Biomed Sci
    關鍵字: Thrombomodulin
    Collective cell migration
    Germ layers formation
    日期: 2019-08
    上傳時間: 2020-07-29 13:53:56 (UTC+8)
    出版者: BMC
    摘要: Background Thrombomodulin (TM), an integral membrane protein, has long been known for its anticoagulant activity. Recent studies showed that TM displays multifaceted activities, including the involvement in cell adhesion and collective cell migration in vitro. However, whether TM contributes similarly to these biological processes in vivo remains elusive. Methods We adapted zebrafish, a prominent animal model for studying molecular/cellular activity, embryonic development, diseases mechanism and drug discovery, to examine how TM functions in modulating cell migration during germ layer formation, a normal and crucial physiological process involving massive cell movement in the very early stages of life. In addition, an in vivo assay was developed to examine the anti-hemostatic activity of TM in zebrafish larva. Results We found that zebrafish TM-b, a zebrafish TM-like protein, was expressed mainly in vasculatures and displayed anti-hemostatic activity. Knocking-down TM-b led to malformation of multiple organs, including vessels, heart, blood cells and neural tissues. Delayed epiboly and incoherent movement of yolk syncytial layer were also observed in early TM-b morphants. Whole mount immunostaining revealed the co-localization of TM-b with both actin and microtubules in epibolic blastomeres. Single-cell tracking revealed impeded migration of blastomeres during epiboly in TM-b-deficient embryos. Conclusion Our results showed that TM-b is crucial to the collective migration of blastomeres during germ layer formation. The structural and functional compatibility and conservation between zebrafish TM-b and mammalian TM support the properness of using zebrafish as an in vivo platform for studying the biological significance and medical use of TM.
    關聯: Journal of Biomedical Science, v.26, n.1, 文獻號碼:60
    Appears in Collections:[藥學系(所)] 期刊論文

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