Sphingosine-1-phosphate in Endothelial Cell Recellularization Improves Patency and Endothelialization of Decellularized Vascular Grafts In Vivo

doi:10.3390/ijms20071641

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Title:	Sphingosine-1-phosphate in Endothelial Cell Recellularization Improves Patency and Endothelialization of Decellularized Vascular Grafts In Vivo
Authors:	Hsia, Kai Lin, Chih-Hsun Lee, Hsin-Yu Chen, Wei-Min Yao, Chao-Ling Mark Chien-Chin Chen(陳建欽) Ma, Hsu Wang, Shyh-Jen Lu, Jen-Her
Contributors:	Natl Taiwan Univ, Dept Life Sci Taipei Vet Gen Hosp, Dept Pediat Taipei Vet Gen Hosp, Div Plast Surg, Dept Surg Natl Yang Ming Univ, Sch Med, Dept Surg Yuan Ze Univ, Dept Chem Engn & Mat Sci, Grad Sch Biotechnol & Bioengn Chia Yi Christian Hosp, Dept Pathol, Ditmanson Med Fdn Chia Nan Univ Pharm & Sci, Dept Cosmet Sci Natl Def Med Ctr, Dept Surg Med & Pediat, Sch Med Taipei Vet Gen Hosp, Div Expt Surg, Dept Surg Natl Yang Ming Univ, Sch Med, Dept Pediat
Keywords:	sphingosine-1-phosphate vascular graft endothelial cell thrombosis syndecan-1
Date:	2019-04
Issue Date:	2020-07-29 13:53:44 (UTC+8)
Publisher:	MDPI
Abstract:	Background: S1P has been shown to improve the endothelialization of decellularized vascular grafts in vitro. Here, we evaluated the potential of tissue-engineered vascular grafts (TEVGs) constructed by ECs and S1P on decellularized vascular scaffolds in a rat model. Methods: Rat aorta was decellularized mainly by 0.1% SDS and characterized by histology. Rat ECs, were seeded onto decellularized scaffolds, and the viability of the ECs was evaluated by biochemical assays. Then, we investigated the in vivo patency rate and endothelialization for five groups of decellularized vascular grafts (each n = 6) in a rat abdominal aorta model for 14 days. The five groups included (1) rat allogenic aorta (RAA); (2) decellularized RAA (DRAA); (3) DRAA with S1P (DRAA/S1P); (4) DRAA with EC recellularization (DRAA/EC); and (5) DRAA with S1P and EC recellularization (DRAA/EC/S1P). Results: In vitro, ECs were identified by the uptake of Dil-Ac-LDL. S1P enhanced the expression of syndecan-1 on ECs and supported the proliferation of ECs on decellularized vascular grafts. In vivo, RAA and DRAA/EC/S1P both had 100% patency without thrombus formation within 14 days. Better endothelialization, more wall structure maintenance and less inflammation were noted in the DRAA/EC/S1P group. In contrast, there was thrombus formation in the DRAA, DRAA/S1P and DRAA/EC groups. Conclusion: S1P could inhibit thrombus formation to improve the patency rate of EC-covered decellularized vascular grafts in vivo and may play an important role in the construction of TEVGs.
Relation:	International Journal of Molecular Sciences, v.20, n.7, 1641
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles

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