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| 標題: | Effect of Vasicinone against Paraquat-Induced MAPK/p53-Mediated Apoptosis via the IGF-1R/PI3K/AKT Pathway in a Parkinson's Disease-Associated SH-SY5Y Cell Model |
| 作者: | Ju, Da-Tong
Sivalingam, Kalaiselvi
Kuo, Wei-Wen
Ho, Tsung-Jung
Chang, Ruey-Lin
Li-Chin Chung(鍾麗琴)
Day, Cecilia Hsuan
Viswanadha, Vijaya Padma
Liao, Po-Hsiang
Huang, Chih-Yang |
| 貢獻者: | Natl Def Med Ctr, Triserv Gen Hosp, Dept Neurol Surg
China Med Univ, China Med Univ Hosp, Dept Med Res
China Med Univ, Dept Biol Sci & Technol, Taichung 404, Taiwan
Tzu Chi Univ, Buddhist Tzu Chi Med Fdn, Hualien Tzu Chi Hosp, Dept Chinese Med
China Med Univ, Coll Chinese Med, Sch Postbaccalaureate Chinese Med
Chia Nan Univ Pharm & Sci, Dept Hosp & Hlth Care Adm
MeiHo Univ, Dept Nursing
Bharathiar Univ, Dept Biotechnol
Hualien Tzu Chi Hosp, Cardiovasc & Mitochondria Related Dis Res Ctr
Tzu Chi Univ Sci & Technol, Buddhist Tzu Chi Med Fdn, Ctr Gen Educ
China Med Univ & Hosp, Grad Inst Biomed
Asia Univ, Dept Biotechnol |
| 關鍵字: | parkinson's disease
vasicinone
paraquat
apoptosis
reactive oxygen species
neuroprotection |
| 日期: | 2019-07 |
| 上傳時間: | 2020-07-29 13:52:41 (UTC+8) |
| 出版者: | MDPI |
| 摘要: | Vasicinone is a quinazoline alkaloid isolated from the Adhatoda vasica plant. In this study, we explored the neuroprotective effect and underlying molecular mechanism of vasicinone against paraquat-induced cellular apoptosis in SH-SY5Y cells. Vasicinone reduced the paraquat-induced loss of cell viability, rescued terminal deoxynucleotide transferase-mediated dUTP nick end-labeling (TUNEL)-positive apoptotic nuclei, and suppressed generation of reactive oxygen species (ROS) in a dose-dependent manner. Western blotting analysis revealed that vasicinone increased the phosphorylation of IGF1R/PI3K/AKT cell survival signaling molecules and downregulated the paraquat-induced, mitogen-activated protein kinase (MAPK)/c-Jun N-terminal kinase (JNK)-mediated apoptotic pathways compared to that observed in cells not treated with vasicinone. This protection depended critically on the activation of IGF1R, and the silencing of IGF1R by siRNA completely abrogated the protective effect of vasicinone in SH-SY5Y cells. Our findings indicated that vasicinone is a potential candidate for the treatment of Parkinson's disease and possibly other oxidative stress-related neurodegenerative disorders. |
| 關聯: | Nutrients, v.11, n.7, 1655 |
| 顯示於類別: | [醫務管理系(所)] 期刊論文
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