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    標題: MiR-338-5p promotes metastasis of colorectal cancer by inhibition of phosphatidylinositol 3-kinase, catalytic subunit type 3-mediated autophagy pathway
    作者: Chu, Chien-An
    Lee, Chung-Ta
    Lee, Jenq-Chang
    Wang, Yi-Wen
    Huang, Ching-Tang
    Lan, Sheng-Hui
    Lin, Peng-Chan
    Lin, Bo-Wen
    Yu-Feng Tian(田宇峯)
    Liu, Hsiao-Sheng
    Chow, Nan-Haw
    貢獻者: Natl Cheng Kung Univ, Coll Med, Inst Basic Med Sci
    Natl Cheng Kung Univ Hosp, Dept Pathol
    Natl Cheng Kung Univ, Coll Med
    Natl Cheng Kung Univ Hosp, Dept Pathol, Dou Liou Branch
    Natl Cheng Kung Univ Hosp, Coll Med, Dept Surg
    Natl Yang Ming Univ, Dept Life Sci
    Natl Yang Ming Univ, Inst Genome Sci
    Natl Cheng Kung Univ, Coll Med, Dept Microbiol & Immunol
    Natl Cheng Kung Univ, Coll Med, Dept Internal Med
    Chia Nan Univ Pharm & Sci, Dept Hlth & Nutr
    Chi Mei Med Ctr, Dept Surg, Div Colorectal Surg
    關鍵字: miR-338-5p
    PIK3C3
    Autophagy and colorectal cancer
    日期: 2019-05
    上傳時間: 2020-07-29 13:52:17 (UTC+8)
    出版者: ELSEVIER SCIENCE BV
    摘要: Background: In our preliminary screening, expression of miR-338-5p was found to be higher in primary colorectal cancer (CRC) with metastasis. The autophagy related gene- phosphatidylinositol 3-kinase, catalytic subunit type 3 (PIK3C3) appeared to be targeted by miR-338-5p. Here, we provide solid evidence in support of PIK3C3 involved in miR-338-5p related metastasis of CRC in vitro and in vivo. Methods: The potential clinical relevance of miR-338-5p and its target gene was analysed on benign colorectal polyps and primary CRCs by QPCR Mouse spleen xenograft experiment was performed to examine the importance of miR-338-5p for metastasis. Findings: PIK3C3 was one of target genes of miR-338-5p. In primary CRCs, expression of miR-338-5p is positively related to tumour staging, distant metastasis and poor patient survival. Patients with higher ratios of miR-338-5p/PIK3C3 also had significantly poor overall survival, supporting their significance in the progression of CRC. Over-expression of miR-338-5p promotes CRC metastasis to the liver and lung in vivo, in which PIK3C3 was down-regulated in the metastatic tumours. In contrast, overexpression of PIK3C3 in miR-338-5p stable cells inhibited the growth of metastatic tumours. Both migration and invasion of CRC in vitro induced by miR-338-5p are mediated by suppression of PIK3C3. Using forward and reverse approaches, autophagy was proved to involve in CRC migration and invasion induced by miR-338-5p. Interpretation: MiR-338-5p induces migration, invasion and metastasis of CRC in part through PIK3C3-related autophagy pathway. The miR-338-5p/PIK3C3 ratio may become a prognostic biomarker for CRC patients. (C) 2019 Published by Elsevier B.V.
    關聯: Ebiomedicine, v.43, pp.270-281
    Appears in Collections:[保健營養系(所) ] 期刊論文

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