Antimicrobial Activity of Lactobacillus Species Against Carbapenem-Resistant Enterobacteriaceae

doi:10.3389/fmicb.2019.00789

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標題:	Antimicrobial Activity of Lactobacillus Species Against Carbapenem-Resistant Enterobacteriaceae
作者:	Chen, Chi-Chung Lai, Chih-Cheng Hui-Ling Huang(黃惠玲) Wen-Yu Huang(黃雯玉) Toh, Han-Siong Weng, Tzu-Chieh Chuang, Yin-Ching Lu, Ying-Chen Tang, Hung-Jen
貢獻者:	Chi Mei Med Ctr, Dept Med Res Natl Chiayi Univ, Dept Food Sci Chi Mei Hosp, Dept Intens Care Med Chia Nan Univ Pharm & Sci, Dept Hlth & Nutr Chi Mei Med Ctr, Dept Intens Care Med Chi Mei Med Ctr, Dept Med
關鍵字:	Lactobacillus carbapenem-resistant Enterobacteriaceae in vitro - antibacterial activity
日期:	2019-04
上傳時間:	2020-07-29 13:52:00 (UTC+8)
出版者:	FRONTIERS MEDIA SA
摘要:	Objective: This study aims to identify suitable lactobacilli that have anti-carbapenemresistant Enterobacteriaceae (CRE) activity with in vitro tolerance to pepsin and bile salts. Methods: Fifty-seven Lactobacillus spp. strains encompassing nine species were collected for investigation. Their viabilities in the presence of pepsin and bile salts were tested using tolerance tests. Their anti-CRE effects were assessed by agar well diffusion and broth microdilution assay, as well as time-kill test. Results: Of the 57 Lactobacillus isolates collected, 31 had a less than 2-log reduction in their viability in both pepsin and bile salt tolerance tests. Of these 31 isolates, 5 (LUC0180, LUC0219, LYC0289, LYC0413, and LYC1031) displayed the greatest anti-CRE activity with a CRE zone of inhibition greater than 15 mm in agar well diffusion assays. The minimal inhibitory percentages of supernatants from these five strains against CREs ranged from 10 to 30%. With the exception of LUC0180, which had a minimal bactericidal percentage >= 40%, the bactericidal percentage of all the strains ranged from 20 to 40%. The inhibitory effect of the cell-free culture supernatants from these Lactobacillus strains did not change after heating but was abolished as the pH changed to 7.0. After a 24-h incubation, five of the Lactobacillus strains at a concentration of 10(8) CFU/ml totally inhibited the growth of carbapenem-resistant Escherichia coil (CRE316) and Kiebsiella pneumoniae (CRE632). After a 48-h incubation, the growth of CRE316 was completely inhibited under each concentration of lactobacilli based on time-kill test. Furthermore, when the concentration of lactobacilli was at 10(8) CFU/ml, the decline in pH was faster than at other concentrations. Conclusion: Some Lactobacillus strains exhibit anti-CRE activity, which suggests potential applications for controlling or preventing CRE colonization or infection.
關聯:	Frontiers in Microbiology, v.10, Article789
顯示於類別:	[保健營養系(所) ] 期刊論文

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